RRC ID 62762
著者 Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H, Okinaga T, Shimizu T, Tominaga K, Nishihara T.
タイトル Lysophosphatidylethanolamine acyltransferase 1/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons.
ジャーナル FASEB J
Abstract Glycerophospholipids, which are components of biomembranes, are formed de novo by the Kennedy pathway and subsequently mature through the Lands cycle. Lysophospholipid acyltransferases (LPLATs) are key enzymes in both pathways and influence the fatty acid composition of biomembranes. Neuronal differentiation is characterized by neurite outgrowth, which requires biomembrane biosynthesis. However, the role of LPLATs in neuronal differentiation remains unknown. In this study, we examined whether LPLATs are involved in neuronal differentiation using all-trans-retinoic acid (ATRA)-treated P19C6 cells. In these cells, mRNA levels of lysophosphatidylethanolamine acyltransferase (LPEAT)-1/membrane-bound O-acyltransferase (MBOAT)-1 were higher than those in undifferentiated cells. LPEAT enzymatic activity increased with 16:0- and 18:1-CoA as acyl donors. When LPEAT1/MBOAT1 was knocked down with small interfering RNA (siRNA), outgrowth of neurites and expression of neuronal markers decreased in ATRA-treated P19C6 cells. Voltage-dependent calcium channel activity was also suppressed in these cells transfected with LPEAT1/MBOAT1 siRNA. These results suggest that LPEAT1/MBOAT1 plays an important role in neurite outgrowth and function.-Tabe, S., Hikiji, H., Ariyoshi, W., Hashidate-Yoshida, T., Shindou, H., Okinaga, T., Shimizu, T., Tominaga, K., Nishihara, T. Lysophosphatidylethanolamine acyltransferase 1/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons.
巻・号 30(7)
ページ 2591-601
公開日 2016-7-1
DOI 10.1096/fj.201500097R
PII fj.201500097R
PMID 27048541
MeSH Acyltransferases / genetics Acyltransferases / metabolism* Animals Cell Differentiation / physiology Cell Line, Tumor Gene Expression Regulation, Enzymologic / physiology* Gene Knockdown Techniques Mice Neurons / cytology* Neurons / drug effects Neurons / physiology RNA, Messenger / genetics RNA, Messenger / metabolism Tretinoin / pharmacology
IF 4.966
リソース情報
ヒト・動物細胞 P19C6(RCB1790)