RRC ID 62772
著者 Suzuki T, Imada T, Nishigaki N, Kobayashi M, Matsuoka I, Kamiya H.
タイトル Cleavage of Target DNA Promotes Sequence Conversion with a Tailed Duplex.
ジャーナル Biol Pharm Bull
Abstract Base sequence conversion in target DNA is achieved when a 5'-tailed duplex (TD) is introduced into cells. In this study, the effects of target DNA cleavage on sequence conversion with a TD were examined. Plasmid DNAs with and without cleavage near the target position were each introduced into HeLa cells, together with the TD. The cleavage promoted the sequence alteration efficiency by ca. 7-fold. These results suggested that the sequence conversion efficiency with the TD fragment is increased when an artificial nuclease introduces cleavage near the target site.
巻・号 39(8)
ページ 1392-5
公開日 2016-1-1
DOI 10.1248/bpb.b16-00325
PMID 27476948
MeSH Base Sequence* DNA / metabolism* DNA Cleavage* HeLa Cells Humans Plasmids
IF 1.863
リソース情報
ヒト・動物細胞 HeLa(RCB0007)