| RRC ID |
62785
|
| 著者 |
Yoshida K, Uehara O, Kurashige Y, Paudel D, Onishi A, Neopane P, Hiraki D, Morikawa T, Harada F, Takai R, Sato J, Saitoh M, Abiko Y.
|
| タイトル |
Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The DNA demethylating agent, 5-Azacytidine (5Aza), and histone deacetylase inhibitor, valproic acid (Vpa), can improve the reprogramming efficiencies of pluripotent cells. This study aimed to examine the roles of 5Aza and Vpa in the dedifferentiation of epithelial cell rests of Malassez (ERM) into stem-like cells. Additionally, the ability of stem-like cells to differentiate into mesenchymal cells was evaluated. ERM was cultured in embryonic stem cell medium (ESCM) with 1 µM of 5Aza, or 2 mM of Vpa, or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells (Pro-DSLCs). The Pro-DSLCs cultured in ESCM alone for another week were named as DSLCs. The stem cell markers were significantly higher in the DSLCs than the controls (no additions). The mRNA and protein levels of the endothelial, mesenchymal stem, and osteogenic cell markers were significantly higher in the Pro-DSLCs and DSLCs than the controls. The combination of a demethylating agent and a deacetylated inhibitor induced the dedifferentiation of ERM into DSLCs. The Pro-DSLCs derived from ERM can be directly reprogrammed into mesenchymal-like cells without dedifferentiation into stem-like cells. Isolated ERM treated with epigenetic agents may be used for periodontal regeneration.
|
| 巻・号 |
11(1)
|
| ページ |
1852
|
| 公開日 |
2021-1-20
|
| DOI |
10.1038/s41598-020-79426-4
|
| PII |
10.1038/s41598-020-79426-4
|
| PMID |
33473142
|
| PMC |
PMC7817677
|
| MeSH |
Animals
Azacitidine / pharmacology*
Cell Dedifferentiation / drug effects
Cell Survival / drug effects
Cellular Reprogramming / drug effects*
Culture Media / chemistry
Epithelial Cells / cytology
Epithelial Cells / metabolism
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / metabolism
Swine
Transcription Factors / genetics
Transcription Factors / metabolism
Valproic Acid / pharmacology*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
253G1(HPS0002) |
