論文 - 詳細
| RRC ID | 62817 |
|---|---|
| 著者 | Oda M, Kurosawa M, Yamamoto H, Domon H, Takenaka S, Ohsumi T, Maekawa T, Yamasaki N, Furue Y, Terao Y. |
| タイトル | Sulfated vizantin inhibits biofilm maturation by Streptococcus mutans. |
| ジャーナル | Microbiol Immunol |
| Abstract |
Streptococcus mutans is the main pathogen of dental caries and adheres to the tooth surface via soluble and insoluble glucans produced by the bacterial glucosyltransferase enzyme. Thus, the S. mutans glucosyltransferase is an important virulence factor for this cariogenic bacterium. Sulfated vizantin effectively inhibits biofilm formation by S. mutans without affecting its growth. In this study, less S. mutans biofilm formation occurred on hydroxyapatite discs coated with sulfated vizantin than on noncoated discs. Sulfated vizantin showed no cytotoxicity against the human gingival cell line Ca9-22. Sulfated vizantin dose-dependently inhibited the extracellular release of cell-free glucosyltransferase from S. mutans and enhanced the accumulation of cell-associated glucosyltransferase, compared with that observed with untreated bacteria. Sulfated vizantin disrupted the localization balance between cell-associated glucosyltransferase and cell-free glucosyltransferase, resulting in inhibited biofilm maturation. These results indicate that sulfated vizantin can potentially serve as a novel agent for preventing dental caries. |
| 巻・号 | 64(7) |
| ページ | 493-501 |
| 公開日 | 2020-7-1 |
| DOI | 10.1111/1348-0421.12797 |
| PMID | 32320095 |
| MeSH | Anti-Bacterial Agents / pharmacology Bacterial Adhesion / drug effects Bacterial Proteins / metabolism Biofilms / drug effects* Biofilms / growth & development* Cell Line Dental Caries / microbiology Dental Caries / prevention & control Glucosyltransferases / antagonists & inhibitors Glucosyltransferases / metabolism Glycolipids / pharmacology* Humans Streptococcus mutans / drug effects* Streptococcus mutans / growth & development* Sulfates / chemistry Trehalose / analogs & derivatives* Trehalose / pharmacology Virulence Factors / metabolism |
| IF | 1.566 |
| リソース情報 | |
| ヒト・動物細胞 | Ca9-22(RCB1976) |