RRC ID 62842
著者 Bracamonte ME, Álvarez AM, Sosa AM, Hoyos CL, Lauthier JJ, Cajal SP, Juarez M, Uncos RE, Sánchez-Valdéz FJ, Acuña L, Diosque P, Basombrío MA, Nasser JR, Hashiguchi Y, Korenaga M, Barroso PA, Marco JD.
タイトル High performance of an enzyme linked immunosorbent assay for American tegumentary leishmaniasis diagnosis with Leishmania (Viannia) braziliensis amastigotes membrane crude antigens.
ジャーナル PLoS One
Abstract The diagnosis of American tegumentary leishmaniasis (ATL) still requires the design of more effective tools. Leishmania (Viannia) braziliensis is the causal agent of the 90% of Argentinean ATL cases. Considering the current knowledge, an ELISA based crude antigen (CA) for the diagnosis was designed. Ninety-nine subjects diagnosed as ATL, 27 as no-ATL, and 84 donors from non-ATL-endemic areas were included in this study. The current ATL diagnosis was based four techniques, dermal smear microscopic examination (parasitological test), PCR, Leishmanin skin test, and clinical records. We obtained CA extracts from promastigotes and amastigotes from macrophage cultures of different zymodemes of endemic Leishmania species circulating in the study area. Crude antigens from the 'local' main zymodeme of L. (V.) braziliensis showed the highest reactivity against anti-Leishmania antibodies compared to the other included species. The CA of amastigotes of this zymodeme was 3.4 fold more reactive than promastigotes one. Moreover, amastigote-membrane CA (MCA) were 3.6 fold more reactive than the soluble antigens. The MCA-ELISA reached a sensitivity and specificity of 98% (CI = 94.7%-100%) and 63.6% (53.9-73.1), respectively. When anti-Trypanosoma cruzi reactive sera were excluded, the specificity reached 98.4% (94.4-100), while the sensitivity was similar, with a positive predictive value (PV) of 98.6% (94.6-100) and negative PV of 96.3% (91.6-100). The performance of the MCA-ELISA results strongly contribute to the final diagnostic decision, since a non-reactive serological result almost discards the suspected ATL, because of its high negative PV. The developed MCA-ELISA showed a high diagnostic performance, which makes it a good candidate for ATL diagnosis, for seroprevalence studies, or for monitoring treatments efficacy.
巻・号 15(5)
ページ e0232829
公開日 2020-1-1
DOI 10.1371/journal.pone.0232829
PII PONE-D-19-33568
PMID 32379842
PMC PMC7205302
MeSH Antibodies, Protozoan / blood* Antibody Affinity Antibody Specificity Antigens, Protozoan / immunology* Argentina / epidemiology Blood Donors Cell Membrane / immunology* Endemic Diseases Enzyme-Linked Immunosorbent Assay / methods* Humans Leishmania braziliensis / growth & development Leishmania braziliensis / immunology* Leishmaniasis, Cutaneous / blood Leishmaniasis, Cutaneous / diagnosis* Leishmaniasis, Cutaneous / epidemiology Leishmaniasis, Cutaneous / parasitology Leishmaniasis, Mucocutaneous / blood Leishmaniasis, Mucocutaneous / diagnosis Leishmaniasis, Mucocutaneous / parasitology Predictive Value of Tests Sensitivity and Specificity Seroepidemiologic Studies Trypanosoma cruzi / immunology
IF 2.74
リソース情報
ヒト・動物細胞 J774.1(RCB0434)