| RRC ID |
62845
|
| 著者 |
Furihata T, Miyaji H, Nishida E, Kato A, Miyata S, Shitomi K, Mayumi K, Kanemoto Y, Sugaya T, Akasaka T.
|
| タイトル |
Bone forming ability of recombinant human collagen peptide granules applied with β-tricalcium phosphate fine particles.
|
| ジャーナル |
J Biomed Mater Res B Appl Biomater
|
| Abstract |
Recombinant human collagen peptide, developed based on human collagen type I, contains an arginyl-glycyl-aspartic acid (RGD)-rich motif to enhance cell behavior and is anticipated as a xeno-free polymer material for use in tissue engineering. We fabricated granules containing recombinant human collagen peptide (RCP) applied with beta-tricalcium phosphate fine particles (RCP/β-TCP) as bone filling scaffold material and assessed the bone forming ability of RCP/β-TCP. Recombinant peptide was thermal crosslinked and freeze-dried to prepare RCP. An aqueous dispersion of β-TCP fine particles was added to RCP to obtain RCP/β-TCP. Subsequently, RCP/β-TCP were characterized using scanning electron microscopy (SEM), energy dispersive X-ray spectrometry (EDX), and cell culture assessments. Furthermore, RCP/β-TCP were implanted into rat cranial bone defects for radiographic and histological evaluations. In SEM and EDX analyses of RCP/β-TCP, β-TCP particles dose-dependently covered the surface of RCP. Cell culture tests showed that RCP/β-TCP remarkably promoted proliferation and mRNA expression of various genes, such as integrin β1 and osteogenic markers, of osteoblastic MC3T3-E1 cells. Histomorphometric assessment at 4 weeks showed that RCP/β-TCP significantly promoted new skull bone formation compared to RCP (p < 0.05) and control (no application) (p < 0.01). Accordingly, these findings suggest RCP/β-TCP possess bone forming capability and would be beneficial for bone tissue engineering therapy.
|
| 巻・号 |
108(7)
|
| ページ |
3033-3044
|
| 公開日 |
2020-10-1
|
| DOI |
10.1002/jbm.b.34632
|
| PMID |
32386261
|
| MeSH |
Animals
Calcium Phosphates* / chemistry
Calcium Phosphates* / pharmacology
Cell Line
Collagen* / chemistry
Collagen* / pharmacology
Humans
Male
Mice
Osteoblasts / metabolism*
Osteogenesis / drug effects*
Peptides* / chemistry
Peptides* / pharmacology
Rats
Rats, Wistar
Recombinant Proteins / chemistry
Recombinant Proteins / pharmacology
|
| IF |
2.831
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
