論文 - 詳細
| RRC ID | 62849 |
|---|---|
| 著者 | Matsumoto A, Asuka M, Takahashi Y, Takakura Y. |
| タイトル | Antitumor immunity by small extracellular vesicles collected from activated dendritic cells through effective induction of cellular and humoral immune responses. |
| ジャーナル | Biomaterials |
| Abstract |
Dendritic cell-derived small extracellular vesicles (DC-sEVs) are proposed as a novel candidate for tumor antigen-based cancer immunotherapy. In order to improve the DC-sEV-induced antitumor immunity, production of DC-sEVs capable of inducing potent antigen-specific humoral and cellular immune responses is necessary. Here, we collected sEVs from DCs and added ovalbumin (OVA), which was used as model antigen, as well as LPS and IFN-γ, to prepare DC-sEVs with high immune activity. After confirming that the collected sEVs, named activated-DCOVA-sEVs, contained OVA and possessed immunologically relevant components (MHC class I molecule displaying antigen epitopes and co-stimulatory molecules, as well as sEV marker proteins), we found that activated-DCOVA-sEV stimulated macrophages and DCs through Toll-like receptor 4 signaling and boosted innate immunity in the tumor microenvironment. Moreover, activated-DCOVA-sEVs induced potent antigen-specific humoral and cellular immune responses both in vitro and in vivo. Finally, immunization with activated-DCOVA-sEVs exhibited stronger in vivo antitumor effects in tumor-bearing mice induced by inoculation with OVA-expressing tumor cells. |
| 巻・号 | 252 |
| ページ | 120112 |
| 公開日 | 2020-9-1 |
| DOI | 10.1016/j.biomaterials.2020.120112 |
| PII | S0142-9612(20)30358-6 |
| PMID | 32422494 |
| MeSH | Animals Dendritic Cells* Extracellular Vesicles* Immunity, Cellular Immunity, Humoral Mice Mice, Inbred C57BL Ovalbumin |
| IF | 10.317 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16/BL6(RCB2638) |