論文 - 詳細
| RRC ID | 62876 |
|---|---|
| 著者 | Kojima H, Itoh T. |
| タイトル | Preparation and Use of Turn-on Fluorescent Probe for Detection and Live Cell Imaging of Vitamin D Receptor as a Target Protein. |
| ジャーナル | STAR Protoc |
| Abstract |
Turn-on fluorescent probe mediated by conjugate addition and cyclization (TCC probe) is a small molecule that reacts with a protein of interest in cells. TCC probe is applicable to various types of proteins by exchanging the ligand unit for target proteins. TCC probes are a potent tool for molecular imaging and chemical proteomics. This protocol describes the synthesis of a TCC probe via unstable intermediate and how to use this probe to visualize vitamin D receptor as a target protein. For complete details on the use and execution of this protocol, please refer to Kojima et al. (2020). |
| 巻・号 | 1(1) |
| ページ | 100036 |
| 公開日 | 2020-6-19 |
| DOI | 10.1016/j.xpro.2020.100036 |
| PII | S2666-1667(20)30023-X |
| PMID | 33111084 |
| PMC | PMC7580106 |
| MeSH | Fluorescent Dyes* / chemical synthesis Fluorescent Dyes* / chemistry Fluorescent Dyes* / metabolism HeLa Cells Humans Molecular Imaging / methods* Optical Imaging / methods* Receptors, Calcitriol* / analysis Receptors, Calcitriol* / chemistry Receptors, Calcitriol* / metabolism |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |