| RRC ID |
62937
|
| 著者 |
De Haven Brandon A, Box G, Hallsworth A, Court W, Matthews N, Herodek B, Arteagabeitia AB, Valenti M, Kirkin V.
|
| タイトル |
Identification of ovarian high-grade serous carcinoma cell lines that show estrogen-sensitive growth as xenografts in immunocompromised mice.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Ovarian cancer remains a significant challenge in women worldwide. Tumors of the high-grade serous carcinoma (HGSC) type represent the most common form of the disease. Development of new therapies for HGSC has been hampered by a paucity of preclinical models in which new drugs could be tested for target engagement and anti-tumor efficacy. Here, we systematically assessed in vivo growth of ovarian cancer cells, including six validated HGSC cell lines, in highly immunocompromised NSG mice by varying the injection site. We found that, with the exception of OVCAR3, HGSC cell lines COV318, COV362, KURAMOCHI, OVCAR4, and OVSAHO, generally demonstrate poor growth as either subcutaneous or intraperitoneal xenografts. Intrabursal injections performed with KURAMOCHI and COV362 cells did not improve tumor growth in vivo. Additional analysis revealed that OVSAHO and COV362 express moderate levels of estrogen receptor (ERα), which translated into improved growth of xenografts in the presence of 17β-Estradiol. Surprisingly, we also found that the growth of the widely used non-HGSC ovarian cell line SKOV3 could be significantly improved by estrogen supplementation. By describing successful establishment of estrogen-sensitive HGSC xenograft models, OVSAHO and COV362, this work will enable testing of novel therapies for this aggressive form of ovarian cancer.
|
| 巻・号 |
10(1)
|
| ページ |
10799
|
| 公開日 |
2020-7-1
|
| DOI |
10.1038/s41598-020-67533-1
|
| PII |
10.1038/s41598-020-67533-1
|
| PMID |
32612269
|
| PMC |
PMC7329846
|
| MeSH |
Animals
Cystadenocarcinoma, Serous / metabolism*
Cystadenocarcinoma, Serous / pathology
Estradiol / metabolism*
Female
Heterografts
Humans
Immunocompromised Host*
Mice
Mice, Inbred NOD
Mice, SCID
Neoplasm Transplantation
Neoplasms, Experimental / metabolism*
Neoplasms, Experimental / pathology
Ovarian Neoplasms / metabolism*
Ovarian Neoplasms / pathology
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
OVK18(RCB1903)
JHOC-5(RCB1520) |
