| RRC ID |
62956
|
| Author |
Bito T, Koseki K, Asano R, Ueda N, Yamada T, Yabuta Y, Ichiyanagi T, Ishihara A, Watanabe K, Watanabe F.
|
| Title |
5-hydroxymethyl-2-furaldehyde purified from Japanese pear (Pyrus pyrifolia Nakai cv. Nijisseiki) juice concentrate inhibits melanogenesis in B16 mouse melanoma cells.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Pear juice concentrate prepared by boiling Japanese pear (Pyrus pyrifolia Nakai cv. Nijisseiki) juice can significantly inhibit the activity of tyrosinase, a key enzyme in melanin synthesis in human skin. Using the ethanol extract of pear juice concentrate, we homogeneously purified an active compound that was identified as 5-hydroxymethyl-2-furaldehyde (5-HMF) through 1H- and 13C-NMR and mass spectroscopy. We observed that 5-HMF inhibited the monophenolase and diphenolase activities of mushroom tyrosinase as a mixed-type inhibitor (Ki values of 3.81 and 3.70 mmol/L, respectively). In B16 mouse melanoma cells, treatment with 170 µmol/L of 5-HMF significantly reduced α-melanocyte-stimulated melanin synthesis by suppressing the cyclic adenosine monophosphate-dependent signaling pathway involved in melanogenesis. The results of our study indicated that 5-HMF can be potentially used as a skin-lightening agent in the cosmetic industry. Abbreviations: AC: adenylate cyclase; CREB: cAMP response element-binding protein; dhFAME: S-(-)-10,11-Dihydroxyfarnesoic acid methyl ester; DMEM: dulbecco's modified eagle medium; l-DOPA: 3-(3,4-Dihydroxyphenyl)- l-alanine; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; HEPES: 4-(2-Hydroxyethyl)-1-piperazine ethane sulfonic acid; 5-HMF: 5-Hydroxymethyl-2-furaldehyde; MITF: microphthalmia-associated transcription factor; α-MSH: α-Melanocyte-stimulating hormone; PKA: protein kinase A; PVDF: polyvinylidene difluoride; SDS: sodium dodecyl sulfate; TRP1: tyrosinase-related protein 1; TRP2: tyrosinase-related protein 2.
|
| Volume |
84(11)
|
| Pages |
2374-2384
|
| Published |
2020-11-1
|
| DOI |
10.1080/09168451.2020.1792762
|
| PMID |
32674671
|
| MeSH |
Animals
Cell Line, Tumor
Fruit and Vegetable Juices / analysis*
Furaldehyde / analogs & derivatives*
Furaldehyde / isolation & purification
Furaldehyde / pharmacology
Melanins / biosynthesis*
Melanoma, Experimental / pathology*
Mice
Monophenol Monooxygenase / antagonists & inhibitors
Oxidoreductases / antagonists & inhibitors
Pyrus / chemistry*
|
| IF |
1.516
|
| Resource |
| Human and Animal Cells |
B16 melanoma(RCB1283) |
