論文 - 詳細
| RRC ID | 62972 |
|---|---|
| 著者 | Akamatsu M, Mashiba R, Kawakatsu K, Ishikawa M, Nishikata T. |
| タイトル | Comparison of Macrophage Activation Using γ-Globulin and Serum-derived Macrophage Activating Factor. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Serum-derived macrophage activating factor (serum-MAF) can rapidly activate macrophage phagocytic activity by inducing characteristic membrane ruffles designated as Frill-like structures. Serum-MAF contains γ-globulin, an activator of phagocytosis. This study examined whether serum-MAF and γ-globulin activate macrophages similarly. MATERIALS AND METHODS:Morphological changes in macrophages were observed by time-lapse imaging and the efficiency of engulfment was analysed quantitatively. Immunological staining of talin-1 and a calpain inhibitor were performed. RESULTS:The engulfment efficiency of serum-MAF- and γ-globulin-activated macrophages was significantly different. Talin-1 showed weak co-localisation with the Frill-like structures. Treatment with a calpain inhibitor similarly down-regulated phagocytosis irrespective of the activation factor. CONCLUSION:There was a difference between macrophage activation mechanisms by γ-globulin and serum-MAF. Talin may slightly contribute to serum-MAF activation. It is possible to distinguish between the calpain-dependent fundamental 'mechanism of phagocytosis' and the activating factor-dependent rapid 'activation mechanism'. |
| 巻・号 | 40(8) |
| ページ | 4707-4710 |
| 公開日 | 2020-8-1 |
| DOI | 10.21873/anticanres.14471 |
| PII | 40/8/4707 |
| PMID | 32727796 |
| MeSH | Calpain / pharmacology Cell Line Down-Regulation / drug effects Humans Macrophage Activation / drug effects* Macrophage-Activating Factors / pharmacology* Macrophages / drug effects* Phagocytosis / drug effects THP-1 Cells gamma-Globulins / pharmacology* |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |