RRC ID 62975
Author Ertunc N, Sato C, Kitajima K.
Title Sialic acid sulfation is induced by the antibiotic treatment in mammalian cells.
Journal Biosci Biotechnol Biochem
Abstract Sialic acids (Sias) are an outermost-situated sugar of glycoproteins and glycolipids to play important roles in various biological phenomena. They are often modified by additional substituents, such as O-acetyl group, to display more than 50 different structures in nature. Of those modified Sia, nothing is known about the occurrence and biological functions of sulfated Sias (SiaSs) in mammals. To elucidate the significance of sialic acid sulfation, we investigated various mammalian-cultured cell lines for the expression of SiaS using the specific antibody 3G9. First, SiaS is expressed in a cell line-dependent and a cell density-dependent manner. Second, in CHO cells, the expression of SiaS is reversibly induced by treatment with the antibiotic G418. Taken together, the expression of SiaS is changed by intrinsic and extrinsic factors in mammalian cells. This is the first demonstration of regulated expression of SiaS.
Volume 84(11)
Pages 2311-2318
Published 2020-11-1
DOI 10.1080/09168451.2020.1792763
PMID 32752946
MeSH Animals Anti-Bacterial Agents / pharmacology* CHO Cells Cell Count Cricetulus Mice N-Acetylneuraminic Acid / metabolism* Sulfates / metabolism*
IF 1.516
Human and Animal Cells HeLa(RCB0007) RAW 264(RCB0535) NB2a(RCB2639) CHO-K1(RCB0285)