| RRC ID |
62996
|
| 著者 |
Ito A, Yoshioka K, Masumoto S, Sato K, Hatae Y, Nakai T, Yamazaki T, Takahashi M, Tanoue S, Horie M.
|
| タイトル |
Magnetic heating of nanoparticles as a scalable cryopreservation technology for human induced pluripotent stem cells.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Scale-up of production is needed for industrial applications and clinical translation of human induced pluripotent stem cells (hiPSCs). However, in cryopreservation of hiPSCs, successful rewarming of vitrified cells can only be achieved by convective warming of small volumes (generally 0.2 mL). Here, we present a scalable nano-warming technology for hiPSC cryopreservation employing inductive heating of magnetic nanoparticles under an alternating magnetic field. The conventional method by water bath heating at 37 °C resulted in a decrease of cell viability owing to devitrification caused by slow warming of samples with large volumes (≥ 20 mL). Nano-warming showed uniform and rapid rewarming of vitrified samples and improved viability of hiPSCs in the 20-mL system. In addition to single cells, hiPSC aggregates prepared using a bioreactor-based approach were successfully cryopreserved by the nano-warming technique. These results demonstrate that nano-warming is a promising methodology for cryopreservation in mass production of hiPSCs.
|
| 巻・号 |
10(1)
|
| ページ |
13605
|
| 公開日 |
2020-8-12
|
| DOI |
10.1038/s41598-020-70707-6
|
| PII |
10.1038/s41598-020-70707-6
|
| PMID |
32788637
|
| PMC |
PMC7423927
|
| MeSH |
Bioreactors
Cell Differentiation
Cell Line
Cell Survival
Convection
Cryopreservation / methods*
Cryoprotective Agents / pharmacology*
Heating / methods*
Humans
Induced Pluripotent Stem Cells / cytology*
Magnetite Nanoparticles
Vitrification
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
201B7(HPS0063) |
