RRC ID 62997
著者 Kikuchi N, Soga T, Nomura M, Sato T, Sakamoto Y, Tanaka R, Abe J, Morita M, Shima H, Okada Y, Tanuma N.
タイトル Comparison of the ischemic and non-ischemic lung cancer metabolome reveals hyper activity of the TCA cycle and autophagy.
ジャーナル Biochem Biophys Res Commun
Abstract Recent advances in cancer biology reveal the importance of metabolic changes in cancer; however, less is known about how metabolic pathways in tumors are regulated in vivo. Here, we report analysis of the lung cancer metabolism based on different surgical procedures, namely lobectomy and partial resection. In lobectomy, but not in partial resection, pulmonary arteries and veins are ligated prior to removal of tissues, rendering tissues ischemic. We show that tumors indeed undergo ischemia upon lobectomy and that the tumor metabolome differs markedly from that of tumors removed by partial resection. Comparison of the responses to ischemia in tumor and normal lung tissues revealed that lung cancer tissue exhibits greater TCA cycle and autophagic activity than do normal lung tissues in vivo in patients. Finally, we report that deleting ATG7, which encodes a protein essential for autophagy, antagonizes growth of tumors derived from lung cancer cell lines, suggesting that autophagy confers metabolic advantages to lung cancer. Our findings shed light on divergent metabolic responses to ischemia seen in tumors and normal tissues.
巻・号 530(1)
ページ 285-291
公開日 2020-9-10
DOI 10.1016/j.bbrc.2020.07.082
PII S0006-291X(20)31471-6
PMID 32828300
MeSH Animals Autophagy Autophagy-Related Protein 7 / genetics Autophagy-Related Protein 7 / metabolism Cell Line, Tumor Citric Acid Cycle* Female Gene Deletion Ischemia / etiology Ischemia / genetics Ischemia / metabolism* Ischemia / pathology Lung Neoplasms / genetics Lung Neoplasms / metabolism* Lung Neoplasms / pathology Lung Neoplasms / surgery* Metabolome* Mice
IF 2.985
リソース情報
ヒト・動物細胞 A549(RCB0098)