論文 - 詳細
| RRC ID | 63012 |
|---|---|
| 著者 | Nakazawa K, Shichino Y, Iwasaki S, Shiina N. |
| タイトル | Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation. |
| ジャーナル | J Biol Chem |
| Abstract |
Regulation of gene expression at the translational level is key to determining cell fate and function. An RNA-binding protein, RNG140 (caprin2), plays a role in eye lens differentiation and has been reported to function in translational regulation. However, the mechanism and its role in eyes has remained unclear. Here, we show that RNG140 binds to the translation initiation factor eukaryotic initiation factor 3 (eIF3) and suppresses translation through mechanisms involving suppression of eIF3-dependent translation initiation. Comprehensive ribosome profiling revealed that overexpression of RNG140 in cultured Chinese hamster ovary cells reduces translation of long mRNAs, including those associated with cell proliferation. RNG140-mediated translational regulation also operates in the mouse eye, where RNG140 knockout increased the translation of long mRNAs. mRNAs involved in lens differentiation, such as crystallin mRNAs, are short and can escape translational inhibition by RNG140 and be translated in differentiating lenses. Thus, this study provides insights into the mechanistic basis of lens cell transition from proliferation to differentiation via RNG140-mediated translational regulation. |
| 巻・号 | 295(44) |
| ページ | 15029-15044 |
| 公開日 | 2020-10-30 |
| DOI | 10.1074/jbc.RA120.012715 |
| PII | S0021-9258(17)48909-5 |
| PMID | 32839273 |
| PMC | PMC7606684 |
| MeSH | Animals CHO Cells Cell Differentiation / physiology* Cell Proliferation / physiology Cricetulus Eukaryotic Initiation Factor-3 / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Lens, Crystalline / cytology Lens, Crystalline / metabolism* Mice Mice, Knockout Protein Biosynthesis / physiology* RNA, Messenger / genetics RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism RNA-Binding Proteins / physiology* |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |