論文 - 詳細
| RRC ID | 63037 |
|---|---|
| 著者 | Kubota H, Hirai T, Yamauchi A, Ogo A, Matsumoto H, Ueno T. |
| タイトル | Inhibitory Effect of Eicosapentaenoic Acid on the Migration of the Esophageal Squamous Cell Carcinoma Cell Line TE-1. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Eicosapentaenoic acid (EPA) inhibits NF-ĸB activation and IL-6 production in TE-1 esophageal cancer cells. NF-ĸB is related to cancer cell migration. The aim of this study is to evaluate whether EPA has a metastasis suppressing effect. Herein, we investigated EPA-treated TE-1 cell migration using TAXIScan. MATERIALS AND METHODS:EZ-TAXIScan® was used to verify whether EPA inhibits cancer cell chemotaxis. RESULTS:Using 50% fetal bovine serum (chemoattractant) without EPA (positive control), average velocity was 0.306±0.084 μm/min compared to 0.162±0.067 μm/min without chemoattraction (negative control). Directionalities of positive and negative controls were 1.039±0.152 and 0.488±0.251 radians, respectively, indicating a significant increase in migration of the positive control compared to that of the negative control. Average velocities were 0.306±0.084 (no EPA), 0.288±0.078 (100 μM EPA), and 0.240±0.054 200 μM (EPA) μm/min, indicating that EPA reduced velocity dose-dependently. Average directionalities were 1.039±0.152 (no EPA), 0.967±0.164 (100 μM EPA), and 0.901±0.146 (200 μM EPA) radians, indicating that EPA also inhibited directionality dose-dependently. CONCLUSION:EPA suppresses directional migration of TE-1 cells. |
| 巻・号 | 40(9) |
| ページ | 5043-5048 |
| 公開日 | 2020-9-1 |
| DOI | 10.21873/anticanres.14507 |
| PII | 40/9/5043 |
| PMID | 32878792 |
| MeSH | Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Cell Line, Tumor Cell Movement / drug effects Chemotaxis / drug effects Eicosapentaenoic Acid / chemistry Eicosapentaenoic Acid / pharmacology* Esophageal Squamous Cell Carcinoma Humans |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | TE-1(RCB1894) |