論文 - 詳細
| RRC ID | 63058 |
|---|---|
| 著者 | Takahashi T, Misawa S, Suzuki S, Saeki N, Shinoda Y, Tsuneoka Y, Akimoto J, Fujiwara Y. |
| タイトル | Possible mechanism of heme oxygenase-1 expression in rat malignant meningioma KMY-J cells subjected to talaporfin sodium-mediated photodynamic therapy. |
| ジャーナル | Photodiagnosis Photodyn Ther |
| Abstract |
BACKGROUND:We previously demonstrated that heme oxygenase-1 (HO-1) induction may contribute to a protective response against photodynamic therapy (PDT) using talaporfin sodium (TS) in rat malignant meningioma KMY-J cells. In the present study, we examined the mechanism of HO-1 induction by PDT with TS (TS-PDT) in KMY-J cells. METHODS:KMY-J cells were incubated with 25 μM TS for 2 h and then exposed to 664 nm diode laser irradiation at 1 J/cm2. The gene and protein expression levels of HO-1 and hypoxia-inducible factor-1α (HIF-1α) were determined by real-time RT-PCR and western blot analysis, respectively. Cell viability was measured using the cell counting kit-8 assay. RESULTS:mRNA and protein levels of HO-1 in KMY-J cells were increased significantly at 3, 6, and 9 h after laser irradiation and the increased mRNA level of HO-1 was decreased by antioxidant N-acetyl cysteine treatment. The protein level of HIF-1α, which mediates transcriptional activation of the HO-1 gene, was increased significantly at 1 h after laser irradiation. Additionally, induction of mRNA expression of HO-1 by TS-PDT was diminished by HIF-1α inhibitor echinomycin. We also demonstrated that echinomycin significantly augmented the cytotoxic effect of TS-PDT. CONCLUSIONS:Our findings indicate that TS-PDT may induce HO-1 expression via reactive oxygen species production and then HIF-1 pathway activation in KMY-J cells, and the HO-1 induction may cause attenuation of the therapeutic effect of TS-PDT. |
| 巻・号 | 32 |
| ページ | 102009 |
| 公開日 | 2020-12-1 |
| DOI | 10.1016/j.pdpdt.2020.102009 |
| PII | S1572-1000(20)30363-X |
| PMID | 32949789 |
| MeSH | Animals Heme Oxygenase-1 Meningeal Neoplasms* Meningioma* / drug therapy Photochemotherapy* / methods Photosensitizing Agents / pharmacology Photosensitizing Agents / therapeutic use Porphyrins Rats |
| IF | 2.894 |
| リソース情報 | |
| ヒト・動物細胞 | KMY-J(RCB1753) |