論文 - 詳細
| RRC ID | 63067 |
|---|---|
| 著者 | Jotatsu T, Izumi H, Morimoto Y, Yatera K. |
| タイトル | Selection of microRNAs in extracellular vesicles for diagnosis of malignant pleural mesothelioma by in vitro analysis. |
| ジャーナル | Oncol Rep |
| Abstract |
Malignant pleural mesothelioma (MPM) is a malignant tumor which is a challenge for diagnosis and is associated with a poor patient prognosis. Thus, early diagnostic interventions will improve the quality of life and life expectancy of these patients. Recently, cellular microRNAs (miRNAs) have been found to be involved in maintaining homeostasis, and abnormal miRNA expression has often been observed in various diseases including cancer. Extracellular vesicles (EVs) released by many cells contain proteins and nucleic acids. miRNAs are secreted from all cells via EVs and circulate throughout the body. In this study, culture media were passed sequentially through membrane filters 220‑50 nm in size, and EVs with diameters of 50 to 220 nm (EVcap50/220) were collected. miRNAs (EV50‑miRNAs) in EVcap50/220 were purified, and microarray analysis was performed. EV50‑miRNA expression profiles were compared between MPM cells and a normal pleural mesothelial cell line, and six EV50‑miRNAs were selected for further investigation. Of these, hsa‑miR‑193a‑5p and hsa‑miR‑551b‑5p demonstrated higher expression in MPM‑derived EVcap50/220. These miRNAs reduced the expression of several genes involved in cell‑cell interactions and cell‑matrix interactions in normal pleural mesothelial cells. Our data suggest that hsa‑miR‑193a‑5p and hsa‑miR‑551b‑5p in EVcap50/220 could be diagnostic markers for MPM. |
| 巻・号 | 44(5) |
| ページ | 2198-2210 |
| 公開日 | 2020-11-1 |
| DOI | 10.3892/or.2020.7778 |
| PMID | 33000251 |
| PMC | PMC7551269 |
| MeSH | Biomarkers, Tumor / analysis* Biomarkers, Tumor / metabolism Cell Line, Tumor Circulating MicroRNA / analysis* Circulating MicroRNA / metabolism Extracellular Vesicles / metabolism Extracellular Vesicles / pathology* Gene Expression Regulation, Neoplastic Humans Liquid Biopsy / methods Mesothelioma, Malignant / blood Mesothelioma, Malignant / diagnosis* Mesothelioma, Malignant / genetics Mesothelioma, Malignant / pathology Pleural Effusion, Malignant / blood Pleural Effusion, Malignant / diagnosis* Pleural Effusion, Malignant / genetics Pleural Effusion, Malignant / pathology |
| IF | 3.417 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) ACC-MESO-4(RCB2293) |