論文 - 詳細
| RRC ID | 63093 |
|---|---|
| 著者 | Inoue Y, Okamoto T, Honda T, Nukui Y, Akashi T, Takemura T, Tozuka M, Miyazaki Y. |
| タイトル | Disruption in the balance between apolipoprotein A-I and mast cell chymase in chronic hypersensitivity pneumonitis. |
| ジャーナル | Immun Inflamm Dis |
| Abstract |
BACKGROUND:Apolipoprotein A-I (apoA-I) has an antifibrotic effect in idiopathic pulmonary fibrosis. Although pulmonary fibrosis is associated with poor prognosis of patients with hypersensitivity pneumonitis (HP), little is known regarding the role of apoA-I in the pathogenesis of HP. METHODS:Two-dimensional electrophoresis, immunoblotting, and enzyme-linked immunosorbent assays were performed for the identification and quantification of apoA-I in bronchoalveolar lavage fluid (BALF) from patients with acute and chronic HP. To investigate the degradation of apoA-I, apoA-I was incubated with BALF. Moreover, the role of apoA-I in TGF-β1-induced epithelial-mesenchymal transition of A549 cells was examined. RESULTS:The concentration of apoA-I in the BALF was significantly lower in chronic HP (n = 56) compared with acute HP (n = 31). The expression level of apoA-I was also low in the lung tissues of chronic HP. ApoA-I was degraded by BALF from HP patients. The number of chymase-positive mast cells in the alveolar parenchyma was inversely correlated with apoA-I levels in the BALF of chronic HP patients. In vitro experiment using A549 cells, untreated apoA-I inhibited TGF-β1-induced epithelial-mesenchymal transition, although this trend was not observed in the chymase-treated apoA-I. CONCLUSIONS:A decrease of apoA-I was associated with the pathogenesis of chronic HP in terms of pulmonary fibrosis and mast cell chymase attenuated the protective effect of apoA-I against pulmonary fibrosis. Furthermore, apoA-I could be a crucial molecule associated with lung fibrogenesis of HP. |
| 巻・号 | 8(4) |
| ページ | 659-671 |
| 公開日 | 2020-12-1 |
| DOI | 10.1002/iid3.355 |
| PMID | 33016012 |
| PMC | PMC7654418 |
| MeSH | Adult Aged Alveolitis, Extrinsic Allergic* Apolipoprotein A-I Bronchoalveolar Lavage Fluid Chymases Female Humans Male Mast Cells* Middle Aged |
| IF | 2.5 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |