| RRC ID |
63099
|
| 著者 |
Nomura S, Takahashi M, Kato AH, Wada Y, Watanabe Y, Yamashita F, Mukai H.
|
| タイトル |
Biosorption-based 64Cu-labeling of bacteria for pharmacokinetic positron-emission tomography.
|
| ジャーナル |
Int J Pharm
|
| Abstract |
Biosorption-based bacterial 64Cu-labeling and its application in pharmacokinetic positron-emission tomography (PET) were investigated. Both gram-positive and gram-negative bacteria were efficiently labeled with [64Cu]Cu2+ ion in saline at room temperature within 5 min. The labeling ratio for Escherichia coli drastically decreased with trypsin pretreatment and the co-presence of excess Cu2+ ion, indicating the existence of specific Cu2+ binding sites on the E. coli cell surface. Washing with lysogeny broth medium was effective in purifying 64Cu-labeled E. coli for kinetic study; the labeling stability was approximately 90% in serum for 15 min. According to dynamic PET imaging in colon-26 tumor-bearing mice, 64Cu-labeled E. coli immediately disappeared from the blood circulation and primarily accumulated in the liver. In addition, transient pulmonary distribution was observed, being in a dose-dependently accelerated manner. Considering the simplicity and versatility of biosorption-based bacterial 64Cu-labeling without genetic modification, the early-phase pharmacokinetic PET with 64Cu-labeled bacteria is promising for assessing toxicological aspects of bacteria-mediated cancer therapy as well as a variety of bacterial pathogenicities in infectious diseases.
|
| 巻・号 |
590
|
| ページ |
119950
|
| 公開日 |
2020-11-30
|
| DOI |
10.1016/j.ijpharm.2020.119950
|
| PII |
S0378-5173(20)30935-2
|
| PMID |
33027635
|
| MeSH |
Animals
Anti-Bacterial Agents*
Cell Line, Tumor
Escherichia coli*
Gram-Negative Bacteria
Gram-Positive Bacteria
Mice
Positron-Emission Tomography
|
| IF |
4.845
|
| リソース情報 |
| ヒト・動物細胞 |
Colon-26(RCB2657) |
| 一般微生物 |
JCM5826
JCM1395
JCM1217 |
