論文 - 詳細
| RRC ID | 63099 |
|---|---|
| 著者 | Nomura S, Takahashi M, Kato AH, Wada Y, Watanabe Y, Yamashita F, Mukai H. |
| タイトル | Biosorption-based 64Cu-labeling of bacteria for pharmacokinetic positron-emission tomography. |
| ジャーナル | Int J Pharm |
| Abstract |
Biosorption-based bacterial 64Cu-labeling and its application in pharmacokinetic positron-emission tomography (PET) were investigated. Both gram-positive and gram-negative bacteria were efficiently labeled with [64Cu]Cu2+ ion in saline at room temperature within 5 min. The labeling ratio for Escherichia coli drastically decreased with trypsin pretreatment and the co-presence of excess Cu2+ ion, indicating the existence of specific Cu2+ binding sites on the E. coli cell surface. Washing with lysogeny broth medium was effective in purifying 64Cu-labeled E. coli for kinetic study; the labeling stability was approximately 90% in serum for 15 min. According to dynamic PET imaging in colon-26 tumor-bearing mice, 64Cu-labeled E. coli immediately disappeared from the blood circulation and primarily accumulated in the liver. In addition, transient pulmonary distribution was observed, being in a dose-dependently accelerated manner. Considering the simplicity and versatility of biosorption-based bacterial 64Cu-labeling without genetic modification, the early-phase pharmacokinetic PET with 64Cu-labeled bacteria is promising for assessing toxicological aspects of bacteria-mediated cancer therapy as well as a variety of bacterial pathogenicities in infectious diseases. |
| 巻・号 | 590 |
| ページ | 119950 |
| 公開日 | 2020-11-30 |
| DOI | 10.1016/j.ijpharm.2020.119950 |
| PII | S0378-5173(20)30935-2 |
| PMID | 33027635 |
| MeSH | Animals Anti-Bacterial Agents* Cell Line, Tumor Escherichia coli* Gram-Negative Bacteria Gram-Positive Bacteria Mice Positron-Emission Tomography |
| IF | 4.845 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Colon-26(RCB2657) |
| 一般微生物 | JCM5826 JCM1395 JCM1217 |