| RRC ID |
63121
|
| 著者 |
Isono T, Domon H, Nagai K, Maekawa T, Tamura H, Hiyoshi T, Yanagihara K, Kunitomo E, Takenaka S, Noiri Y, Terao Y.
|
| タイトル |
Treatment of severe pneumonia by hinokitiol in a murine antimicrobial-resistant pneumococcal pneumonia model.
|
| ジャーナル |
PLoS One
|
| Abstract |
Streptococcus pneumoniae is often isolated from patients with community-acquired pneumonia. Antibiotics are the primary line of treatment for pneumococcal pneumonia; however, rising antimicrobial resistance is becoming more prevalent. Hinokitiol, which is isolated from trees in the cypress family, has been demonstrated to exert antibacterial activity against S. pneumoniae in vitro regardless of antimicrobial resistance. In this study, the efficacy of hinokitiol was investigated in a mouse pneumonia model. Male 8-week-old BALB/c mice were intratracheally infected with S. pneumoniae strains D39 (antimicrobial susceptible) and NU4471 (macrolide resistant). After 1 h, hinokitiol was injected via the tracheal route. Hinokitiol significantly decreased the number of S. pneumoniae in the bronchoalveolar lavage fluid (BALF) and the concentration of pneumococcal DNA in the serum, regardless of whether bacteria were resistant or susceptible to macrolides. In addition, hinokitiol decreased the infiltration of neutrophils in the lungs, as well as the concentration of inflammatory cytokines in the BALF and serum. Repeated hinokitiol injection at 18 h intervals showed downward trend in the number of S. pneumoniae in the BALF and the concentration of S. pneumoniae DNA in the serum with the number of hinokitiol administrations. These findings suggest that hinokitiol reduced bacterial load and suppressed excessive host immune response in the pneumonia mouse model. Accordingly, hinokitiol warrants further exploration as a potential candidate for the treatment of pneumococcal pneumonia.
|
| 巻・号 |
15(10)
|
| ページ |
e0240329
|
| 公開日 |
2020-1-1
|
| DOI |
10.1371/journal.pone.0240329
|
| PII |
PONE-D-20-12596
|
| PMID |
33057343
|
| PMC |
PMC7561173
|
| MeSH |
Animals
Anti-Infective Agents / pharmacology*
Anti-Infective Agents / therapeutic use
Bronchoalveolar Lavage Fluid / microbiology
Chemokine CXCL1 / blood
Chemokine CXCL1 / metabolism
Cytokines / blood
Cytokines / metabolism
Drug Resistance, Bacterial
Interleukin-6 / blood
Interleukin-6 / metabolism
Lung / immunology
Lung / pathology
Male
Mice
Mice, Inbred BALB C
Monoterpenes / pharmacology*
Monoterpenes / therapeutic use
Neutrophil Infiltration
Neutrophils / cytology
Neutrophils / immunology
Neutrophils / metabolism
Pneumonia, Pneumococcal / drug therapy
Pneumonia, Pneumococcal / microbiology
Pneumonia, Pneumococcal / pathology*
Streptococcus pneumoniae / isolation & purification*
Streptococcus pneumoniae / pathogenicity
Tropolone / analogs & derivatives*
Tropolone / pharmacology
Tropolone / therapeutic use
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
A549 |
