論文 - 詳細
RRC ID | 63127 |
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著者 | Nishigaki C, Nakayama M, Miyata H. |
タイトル | Cell physiological responses of RAW264 macrophage cells to a 50-Hz magnetic field. |
ジャーナル | Int J Radiat Biol |
Abstract |
PURPOSE:We previously showed that a 0.5-mT, 50-Hz sinusoidal magnetic field (LFMF) enhanced DNA single-strand breaks (SSB) and necrosis in RAW264 macrophages that had been stimulated by bacterial endotoxin (lipopolysaccharide; LPS). LPS enhances production of nitric oxide (NO) and superoxide anion (O2-) that react with each other to generate peroxynitrite (ONOO-). ONOO- causes DNA strand breaks. Hence, we anticipated that 0.5-mT, 50-Hz sinusoidal magnetic field increased production of NO, thereby increasing intracellular ONOO- concentration and promoted DNA strand breaks. However, the NO production was not increased. In this study, we examined if the exposure of the cell to 0.5-mT, 50-Hz magnetic field for 24 h (1) promotes O2- production, (2) elevated the degree of apoptosis, because apoptosis is an upstream event of necrosis, (3) lowers mitochondrial membrane potential (ΔΦm), because it would also promote necrosis. MATERIALS AND METHODS:O2-, was measured with nitroblue tetrazolium and water-soluble tetrazolium salt. Necrosis and apoptosis were quantified with propidium iodide and fluorescence labelling of caspases, respectively. The ΔΦm was measured with a fluorescent probe (JC-1) that reflects ΔΦm. Results and conclusions: In the LPS-stimulated macrophage, the LFMF did not promote O2- production. Thus, the LFMF-promoted DNA strand breaks did not result from the increase in the O2- production. The LFMF did not promote apoptosis, whereas it tended to increase the degree of necrosis, as we showed previously. The ΔΦm slightly declined in the LFMF-exposed cell without statistical significance. |
巻・号 | 96(12) |
ページ | 1628-1632 |
公開日 | 2020-12-1 |
DOI | 10.1080/09553002.2020.1837983 |
PMID | 33052723 |
MeSH | Animals Apoptosis Macrophages / cytology* Magnetic Fields* Membrane Potential, Mitochondrial Mice RAW 264.7 Cells |
IF | 2.368 |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |