論文 - 詳細
| RRC ID | 63144 |
|---|---|
| 著者 | Sakuda T, Kubo T, Johan MP, Furuta T, Sakaguchi T, Adachi N. |
| タイトル | Development of an Oncolytic Recombinant Vesicular Stomatitis Virus Encoding a Tumor-suppressor MicroRNA. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:Attempts have been made to enhance systemic therapy for osteosarcoma. In our previous study, the systemic administration of a vesicular stomatitis virus (VSV) improved the survival rates of mice with osteosarcoma but did not improve the long-term survival of the animals. MATERIALS AND METHODS:In the present study, we developed a novel oncolytic VSV by incorporating tumor-suppressor microRNA143 (rVSV-miR143) to compare the antitumor effects of various doses (10×10-4, 5×10-4, and 1×10-4 multiplicity of infection) of rVSV-miR143 with those of VSV in vitro. RESULTS:The cytotoxicity and migration-inhibitory effects of rVSV-miR143 on the osteosarcoma cells were significantly higher than those of VSV alone at a dose of 5×10-4 multiplicity of infection, indicating that rVSV-miRNA143 enhances the antitumor effect at certain doses. CONCLUSION:VSV incorporating tumor-suppressor miRNA143 demonstrated a synergistic antitumor effect on osteosarcoma cells in vitro. |
| 巻・号 | 40(11) |
| ページ | 6319-6325 |
| 公開日 | 2020-11-1 |
| DOI | 10.21873/anticanres.14652 |
| PII | 40/11/6319 |
| PMID | 33109569 |
| MeSH | Animals Cell Line, Tumor Cell Movement / genetics Gene Expression Regulation, Neoplastic Genes, Tumor Suppressor* Humans Mice MicroRNAs / genetics* MicroRNAs / metabolism Oncolytic Virotherapy Oncolytic Viruses / genetics* Osteosarcoma / genetics Osteosarcoma / pathology Recombination, Genetic / genetics* Vesicular stomatitis Indiana virus / genetics* |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | LM8(RCB1450) |