論文 - 詳細
| RRC ID | 63195 |
|---|---|
| 著者 | Imoto Y, Takabayashi T, Sakashita M, Kato Y, Yoshida K, Kidoguchi M, Koyama K, Adachi N, Kimura Y, Ogi K, Ito Y, Kanno M, Okamoto M, Narita N, Fujieda S. |
| タイトル | Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis. |
| ジャーナル | Biomolecules |
| Abstract |
BACKGROUND:The pathological features of chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) tissues include an eosinophilic infiltration pattern (eosinophilic CRS (ECRS)) or a less eosinophilic pattern (non-ECRS). Recently, it has been suggested that 15-lipoxygenase 1 (15-LOX-1) may have significant roles in allergic disease; however, the significance of 15-LOX-1 in CRS is not well understood. The objective of this study was to demonstrate the expression of 15-LOX-1 in CRS. METHODS:The mRNA expression levels of 15-LOX-1 and periostin in nasal tissues were measured by quantitative real-time polymerase chain reaction. We also performed an immunofluorescence study of nasal tissues. Cells of the Eol-1 eosinophilic leukemic cell line were stimulated with interleukin-33 to test the induction of 15-LOX-1. RESULTS:The expression level of 15-LOX-1 mRNA in nasal polyps (NPs) was significantly higher in ECRS patients than in non-ECRS patients. The immunofluorescence study revealed that both airway epithelial cells and eosinophils in NPs expressed 15-LOX-1. A significant correlation was seen between the number of eosinophils and the mRNA expression levels of 15-LOX-1 and periostin in nasal polyps. Moreover, interleukin-33 enhanced 15-LOX-1 expression in Eol-1 cells. CONCLUSIONS:15-LOX-1 was shown to be a significant molecule that facilitates eosinophilic inflammation in ECRS. |
| 巻・号 | 10(11) |
| 公開日 | 2020-11-18 |
| DOI | 10.3390/biom10111568 |
| PII | biom10111568 |
| PMID | 33218117 |
| PMC | PMC7698943 |
| MeSH | Adult Aged Arachidonate 15-Lipoxygenase / biosynthesis* Arachidonate 15-Lipoxygenase / genetics Cell Adhesion Molecules / biosynthesis* Cell Adhesion Molecules / genetics Chronic Disease Eosinophils / metabolism* Eosinophils / pathology Female Gene Expression Humans Male Middle Aged Rhinorrhea / genetics Rhinorrhea / metabolism* Rhinorrhea / pathology Sinusitis / genetics Sinusitis / metabolism* Sinusitis / pathology |
| IF | 4.082 |
| リソース情報 | |
| ヒト・動物細胞 | EoL-1 cell(RCB0641) |