RRC ID 63201
著者 Hasegawa T, Takenaka S, Oda M, Domon H, Hiyoshi T, Sasagawa K, Ohsumi T, Hayashi N, Okamoto Y, Yamamoto H, Ohshima H, Terao Y, Noiri Y.
タイトル Sulfated vizantin causes detachment of biofilms composed mainly of the genus Streptococcus without affecting bacterial growth and viability.
ジャーナル BMC Microbiol
Abstract BACKGROUND:Sulfated vizantin, a recently developed immunostimulant, has also been found to exert antibiofilm properties. It acts not as a bactericide, but as a detachment-promoting agent by reducing the biofilm structural stability. This study aimed to investigate the mechanism underlying this activity and its species specificity using two distinct ex vivo oral biofilm models derived from human saliva.
RESULTS:The biofilm, composed mainly of the genus Streptococcus and containing 50 μM of sulfated vizantin, detached significantly from its basal surface with rotation at 500 rpm for only 15 s, even when 0.2% sucrose was supplied. Expression analyses for genes associated with biofilm formation and bacterial adhesion following identification of the Streptococcus species, revealed that a variety of Streptococcus species in a cariogenic biofilm showed downregulation of genes encoding glucosyltransferases involved in the biosynthesis of water-soluble glucan. The expression of some genes encoding surface proteins was also downregulated. Of the two quorum sensing systems involved in the genus Streptococcus, the expression of luxS in three species, Streptococcus oralis, Streptococcus gordonii, and Streptococcus mutans, was significantly downregulated in the presence of 50 μM sulfated vizantin. Biofilm detachment may be facilitated by the reduced structural stability due to these modulations. As a non-specific reaction, 50 μM sulfated vizantin decreased cell surface hydrophobicity by binding to the cell surface, resulting in reduced bacterial adherence.
CONCLUSION:Sulfated vizantin may be a candidate for a new antibiofilm strategy targeting the biofilm matrix while preserving the resident microflora.
巻・号 20(1)
ページ 361
公開日 2020-11-25
DOI 10.1186/s12866-020-02033-w
PII 10.1186/s12866-020-02033-w
PMID 33238885
PMC PMC7687742
MeSH Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology* Bacterial Adhesion / drug effects Bacterial Adhesion / genetics Bacterial Proteins / genetics Bacterial Proteins / metabolism Biofilms / drug effects* Biofilms / growth & development Dental Caries / microbiology Epithelial Cells / drug effects Gene Expression / drug effects Gingivitis / microbiology Glucosyltransferases / genetics Glucosyltransferases / metabolism Glycolipids / chemistry Glycolipids / pharmacology* Humans Quorum Sensing / drug effects Quorum Sensing / genetics Streptococcus / classification Streptococcus / drug effects Streptococcus / growth & development Streptococcus / physiology* Sulfates / chemistry Trehalose / analogs & derivatives* Trehalose / chemistry Trehalose / pharmacology
IF 2.989
リソース情報
ヒト・動物細胞 Ca9-22(RCB1976)