論文 - 詳細
| RRC ID | 63206 |
|---|---|
| 著者 | Yamaguchi K, Otomo K, Kozawa Y, Tsutsumi M, Inose T, Hirai K, Sato S, Nemoto T, Uji-I H. |
| タイトル | Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens. |
| ジャーナル | ACS Omega |
| Abstract |
We developed adaptive optical (AO) two-photon excitation microscopy by introducing a spatial light modulator (SLM) in a commercially available microscopy system. For correcting optical aberrations caused by refractive index (RI) interfaces at a specimen's surface, spatial phase distributions of the incident excitation laser light were calculated using 3D coordination of the RI interface with a 3D ray-tracing method. Based on the calculation, we applied a 2D phase-shift distribution to a SLM and achieved the proper point spread function. AO two-photon microscopy improved the fluorescence image contrast in optical phantom mimicking biological specimens. Furthermore, it enhanced the fluorescence intensity from tubulin-labeling dyes in living multicellular tumor spheroids and allowed successful visualization of dendritic spines in the cortical layer V of living mouse brains in the secondary motor region with a curved surface. The AO approach is useful for observing dynamic physiological activities in deep regions of various living biological specimens with curved surfaces. |
| 巻・号 | 6(1) |
| ページ | 438-447 |
| 公開日 | 2021-1-12 |
| DOI | 10.1021/acsomega.0c04888 |
| PMID | 33458495 |
| PMC | PMC7807736 |
| IF | 2.87 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |