| RRC ID |
63232
|
| 著者 |
Lai YH, Lee PY, Lu CY, Liu YR, Wang SC, Liu CC, Chang YC, Chen YH, Su CC, Li CY, Liu PL.
|
| タイトル |
Thrombospondin 1-induced exosomal proteins attenuate hypoxia-induced paraptosis in corneal epithelial cells and promote wound healing.
|
| ジャーナル |
FASEB J
|
| Abstract |
Thrombospondin-1 (TSP1) is involved in corneal wound healing caused by chemical injury. Herein, we examined the effects of TSP1 on hypoxia-induced damages and wound-healing activity in human corneal epithelial (HCE) cells. Exosomal protein expression was determined using liquid chromatography-tandem mass spectrometry, and HCE cell migration and motility were examined through wound-healing assay and time-lapse microscopy. Reestablishment of cell junctions by TSP1 was assessed through confocal microscopy and 3D image reconstruction. Our results show that CoCl2 -induced hypoxia promoted HCE cell death by paraptosis. TSP1 protected these cells against paraptosis by attenuating mitochondrial membrane potential depletion, swelling and dilation of endoplasmic reticulum and mitochondria, and mitochondrial fission. Exosomes isolated from HCE cells treated with TSP1 contained wound healing-associated proteins that were taken up by HCE cells to promote tissue remodeling and repair. TSP1 protected HCE cells against hypoxia-induced damages and inhibited paraptosis progression by promoting cell migration, cell-cell adhesion, and extracellular matrix remodeling. These findings indicate that TSP1 ameliorates hypoxia-induced paraptosis in HCE cells and promotes wound healing and remodeling by regulating exosomal protein expression. TSP1 may, therefore, play important roles in the treatment of hypoxia-associated corneal diseases.
|
| 巻・号 |
35(1)
|
| ページ |
e21200
|
| 公開日 |
2021-1-1
|
| DOI |
10.1096/fj.202001106RRR
|
| PMID |
33341997
|
| MeSH |
Cell Hypoxia / drug effects
Cell Line
Cobalt / pharmacology
Endoplasmic Reticulum / metabolism
Epithelial Cells / metabolism*
Epithelial Cells / pathology
Epithelium, Corneal / metabolism*
Epithelium, Corneal / pathology
Exosomes / metabolism*
Exosomes / pathology
Humans
Membrane Potential, Mitochondrial / drug effects
Mitochondria / metabolism
Mitochondria / pathology
Mitochondrial Membranes / metabolism
Thrombospondin 1 / metabolism*
Wound Healing*
|
| IF |
4.966
|
| リソース情報 |
| ヒト・動物細胞 |
HCE-T(RCB2280) |
