RRC ID 63237
著者 Frazer H, You J, Chen Z, Sayyar S, Liu X, Taylor A, Hodge C, Wallace G, Sutton G.
タイトル Development of a Platelet Lysate-Based Printable, Transparent Biomaterial With Regenerative Potential for Epithelial Corneal Injuries.
ジャーナル Transl Vis Sci Technol
Abstract Purpose:To develop a human platelet lysate (hPL)-based bioink that is transparent and also encompasses the regenerative properties of hPL to facilitate wound healing.
Methods:The effect of different batches of hPLand fetal bovine serum (FBS) on corneal epithelial cell growth and scratch assay was first examined using Incucyte Zoom. Various combinations of human fibrinogen (concentration range from 0.2 to 5 mg/mL) and thrombin (concentration from 1 to 10 U/mL) were combined with hPL to generate nine types of potential bioink. Rheology, transparency, and cell compatibility of bioinks were assessed and compared. The final selected bioink was used in an ex vivo corneal model to examine its ability in re-epithelization.
Results:No significant difference was detected in cell proliferation and wound healing tests between different hPL batches at the same concentration. Scratch assay data showed that hPL had significantly higher effect on wound healing than FBS. Comparing across the nine bioinks, bioink 5 consisting of 10% hPL, 2 mg/mL fibrinogen, and 5 U/mL thrombin demonstrated all required mechanical and cellular properties and was able to regenerate the full-thickness epithelium ex vivo.
Conclusions:The results showed that a transparent and adhesive bioink can be generated by combining hPL, fibrinogen, and thrombin together. The bioink can be directly applied to a human cornea to promote corneal re-epithelization with huge potential applications in corneal injuries.
Translational Relevance:The developed transparent hPL-based ink with its adhesive and healing ability showed that it could be used as a new treatment option for corneal injuries.
巻・号 9(13)
ページ 40
公開日 2020-12-1
DOI 10.1167/tvst.9.13.40
PII TVST-20-2974
PMID 33442494
PMC PMC7779874
MeSH Biocompatible Materials* Blood Platelets Cell Proliferation Corneal Injuries* / therapy Humans Wound Healing
IF 2.112
リソース情報
ヒト・動物細胞 HCE-T(RCB2280)