| RRC ID |
63242
|
| Author |
Fujiwara Y, Murofushi T, Koshi R, Mikami Y, Tsuda H.
|
| Title |
Reactive oxygen species-dependent release of damage-associated molecular patterns from human gingival epithelial Ca9-22 cells during butyrate or propionate exposure.
|
| Journal |
J Oral Sci
|
| Abstract |
Treating the gingival epithelial Ca9-22 cell with butyrate, a short-chain fatty acid (SCFA) produced by bacteria within mature dental plaque, induces necrotic cellular death. In this report, it was examined whether SCFA-mediated cellular death is accompanied by a release of damage-associated molecular patterns (DAMPs). In addition, the role of reactive oxygen species (ROS) in the release of DAMPs was evaluated. Human gingival epithelial Ca9-22 cells were treated with butyrate or propionate. The amounts of dead cells were then measured using SYTOX-green dye. Released DAMPs were detected by western blot. The role of ROS scavengers, ascorbic acid and N-acetylcysteine, on DAMP-release was evaluated. Dose and time-dependent induction of Ca9-22 cell death was observed during butyrate and propionate treatments. This was accompanied by the release of DAMPs. Ascorbic acid or N-acetylcysteine reduced cellular death and inhibited DAMP-release induced by exposure to butyrate or propionate. These data collectively suggest that SCFA-induced death of gingival epithelial Ca9-22 cells and accompanying release of DAMPs are dependent on ROS.
|
| Volume |
63(2)
|
| Pages |
195-197
|
| Published |
2021-3-31
|
| DOI |
10.2334/josnusd.20-0411
|
| PMID |
33390461
|
| MeSH |
Antigens, Neoplasm
Butyrates* / pharmacology
Carbonic Anhydrase IX
Epithelial Cells
Gingiva
Humans
Propionates* / pharmacology
Reactive Oxygen Species
|
| IF |
1.2
|
| Resource |
| Human and Animal Cells |
Ca9-22(RCB1976) |
