Reference - Detail
| RRC ID | 63242 |
|---|---|
| Author | Fujiwara Y, Murofushi T, Koshi R, Mikami Y, Tsuda H. |
| Title | Reactive oxygen species-dependent release of damage-associated molecular patterns from human gingival epithelial Ca9-22 cells during butyrate or propionate exposure. |
| Journal | J Oral Sci |
| Abstract |
Treating the gingival epithelial Ca9-22 cell with butyrate, a short-chain fatty acid (SCFA) produced by bacteria within mature dental plaque, induces necrotic cellular death. In this report, it was examined whether SCFA-mediated cellular death is accompanied by a release of damage-associated molecular patterns (DAMPs). In addition, the role of reactive oxygen species (ROS) in the release of DAMPs was evaluated. Human gingival epithelial Ca9-22 cells were treated with butyrate or propionate. The amounts of dead cells were then measured using SYTOX-green dye. Released DAMPs were detected by western blot. The role of ROS scavengers, ascorbic acid and N-acetylcysteine, on DAMP-release was evaluated. Dose and time-dependent induction of Ca9-22 cell death was observed during butyrate and propionate treatments. This was accompanied by the release of DAMPs. Ascorbic acid or N-acetylcysteine reduced cellular death and inhibited DAMP-release induced by exposure to butyrate or propionate. These data collectively suggest that SCFA-induced death of gingival epithelial Ca9-22 cells and accompanying release of DAMPs are dependent on ROS. |
| Volume | 63(2) |
| Pages | 195-197 |
| Published | 2021-3-31 |
| DOI | 10.2334/josnusd.20-0411 |
| PMID | 33390461 |
| MeSH | Antigens, Neoplasm Butyrates* / pharmacology Carbonic Anhydrase IX Epithelial Cells Gingiva Humans Propionates* / pharmacology Reactive Oxygen Species |
| IF | 1.2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Ca9-22(RCB1976) |