RRC ID 63263
Author Sekiya M, Kiyohara H, Maruyama H, Yabe T, Yamada H.
Title Modulation of chemokine expression on intestinal epithelial cells by Kampo (traditional Japanese herbal) medicine, Hochuekkito, and its active ingredients.
Journal J Nat Med
Abstract The intestinal epithelial cells sit at the interface between a lumen and a lamina propria or lymph nodes such as Peyer's patches, where they play important roles in maintaining intestinal homeostasis through chemokine secretion. This study investigated the effect of Hochuekkito (TJ-41)-a traditional Japanese herbal (Kampo) formula used as a tonic for weakness-on chemokine expression in intestinal epithelial cells in order to explore the mechanism of its modulating effect against mucosal immunity. When cells from the rat normal small intestinal epithelial cell-line IEC-6 were stimulated with TJ-41, mRNA expression of CC chemokine ligand (CCL) 11 (eotaxin), CCL20 (MIP-3α) and CCL25 (TECK) was enhanced. Oral administration of TJ-41 to methotrexate-treated mice enhanced mRNA expression of CCL25 and keratinocyte growth factor in the jejunum with, decreasing mRNA expression of the inflammatory marker tumor necrosis factor (TNF)-α. Although oral administration of TJ-41 did not affect CCL20 mRNA expression in villus epithelium of methotrexate-treated mice, enhancement of CCL20 mRNA expression was observed in Peyer's patches. Immunohistochemical analysis detected dense staining with anti-CCL20 antibody in the follicle-associated epithelium region of Peyer's patches in mice administered TJ-41. Analysis of active ingredients indicates that polysaccharide-containing macromolecules in TJ-41 contribute to the enhancement of CCL20 mRNA expression through an intracellular signal cascade via nuclear factor kappa B (NF-κB) activation.
Volume 67(3)
Pages 626-35
Published 2013-7-1
DOI 10.1007/s11418-012-0724-1
PMID 23203627
MeSH Administration, Oral Animals Anti-Inflammatory Agents / administration & dosage Anti-Inflammatory Agents / pharmacology* Cell Line Chemokine CCL11 / metabolism Chemokine CCL20 / metabolism Chemokines / genetics Chemokines / metabolism* Chemokines, CC / metabolism Drugs, Chinese Herbal / administration & dosage Drugs, Chinese Herbal / pharmacology* Epithelial Cells / drug effects* Epithelial Cells / immunology Epithelial Cells / metabolism Female Fibroblast Growth Factor 7 / metabolism Intestinal Mucosa / drug effects* Intestinal Mucosa / immunology Intestinal Mucosa / metabolism Jejunum / drug effects* Jejunum / immunology Jejunum / metabolism Medicine, Kampo* Mice Mice, Inbred BALB C NF-kappa B / metabolism Peyer's Patches / drug effects Peyer's Patches / metabolism Phytotherapy Plants, Medicinal RNA, Messenger / metabolism Rats Signal Transduction Time Factors Tumor Necrosis Factor-alpha / metabolism
IF 2.055
Human and Animal Cells IEC 6(RCB0993)