RRC ID 63265
Author Kimura O, Tsukagoshi K, Hayasaka M, Endo T.
Title Uptake of triclopyr (3,5,6-trichloro-2-pyridinyloxyacetic acid) and dicamba (3,6-dichloro-2-methoxybenzoic acid) from the apical membranes of the human intestinal Caco-2 cells.
Journal Arch Toxicol
Abstract We investigated whether the uptake of triclopyr (3, 5, 6-trichloro-2-pyridinyloxyacetic acid) and dicamba (3,6-dichloro-2-methoxybenzoic acid) across the apical membrane of Caco-2 cells was mediated via proton-linked monocarboxylic acid transporters (MCTs). The uptake of triclopyr from the apical membranes was fast, pH-, temperature-, and concentration dependent, required metabolic energy to proceed, and was competitively inhibited by monocarboxylic acids such as benzoic acid and ferulic acid (substrates of L-lactic acid-insensitive MCTs), but not by L-lactic acid. Thus, the uptake of triclopyr in Caco-2 cells appears to be mediated mainly via L-lactic acid-insensitive MCTs. In contrast, the uptake of dicamba (a benzoic acid derivative) was slow, and it was both pH- and temperature dependent. Coincubation with ferulic acid did not decrease the uptake of dicamba, although coincubation with benzoic acid moderately decreased it. The uptake of dicamba appears to be mediated mainly via passive diffusion, which is in contrast to the uptake of benzoic acid via MCTs. We speculate that the substituted groups in dicamba may inhibit uptake via MCTs.
Volume 86(1)
Pages 55-61
Published 2012-1-1
DOI 10.1007/s00204-011-0734-x
PMID 21766207
MeSH Benzoic Acid / chemistry Biological Transport Caco-2 Cells Coumaric Acids / chemistry Dicamba / administration & dosage Dicamba / pharmacokinetics* Dose-Response Relationship, Drug Glycolates / administration & dosage Glycolates / pharmacokinetics* Herbicides / administration & dosage Herbicides / pharmacokinetics* Humans Hydrogen-Ion Concentration Intestinal Absorption Monocarboxylic Acid Transporters / metabolism* Temperature Time Factors
IF 5.059
Human and Animal Cells CAC0-2(RCB0988)