RRC ID 63315
Author Inui K, Ito H, Miyata K, Matsuo T, Horiuchi R, Ikeda T, Watanabe T, Ohyama T, Niwa K.
Title Involvement of sialic acid in transport of serotype C1 botulinum toxins through rat intestinal epithelial cells.
Journal J Vet Med Sci
Abstract Clostridium botulinum produces a large toxin complex (L-TC) composed of neurotoxin (BoNT) and non-toxic proteins. In animal botulism, BoNT or L-TC is absorbed via the intestinal epithelium. To establish the cellular mechanisms of botulinum toxin absorption, we used cultured rat intestinal epithelial cells to test the binding and transport of serotype C1 BoNT and L-TC through the cell layers. BoNT and L-TC bound to and passed through the cell layers, with L-TC exhibiting larger binding and transport. Binding and transport of these toxins were inhibited by N-acetyl neuraminic acid or neuraminidase treatment of the cells. These results suggest that binding of serotype C1 BoNT and L-TC to sialic acid on the cells promoted their transport through intestinal epithelial cell layers.
Volume 72(9)
Pages 1251-5
Published 2010-9-1
DOI 10.1292/jvms.10-0090
PII JST.JSTAGE/jvms/10-0090
PMID 20453451
MeSH Animals Binding Sites Biological Transport Botulinum Toxins / metabolism* Epithelial Cells / metabolism* Galactose / metabolism Intestinal Mucosa / metabolism Kinetics Lactose / metabolism N-Acetylneuraminic Acid / metabolism* Neuraminic Acids / metabolism Neurotoxins / metabolism Poly(ADP-ribose) Polymerases / metabolism Rats
IF 1.049
Human and Animal Cells IEC 6(RCB0993)