Reference - Detail
| RRC ID | 63333 |
|---|---|
| Author | Hideaki Shiraishi, Akiko Toyoda |
| Title | The use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide–based colorimetric assay in the viability analysis of the filamentous cyanobacterium Arthrospira platensis |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Abstract |
The applicability of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay to an industrially valuable filamentous cyanobacterium Arthrospira platensis was examined. When it was applied to A. platensis NIES-39, as few as 10 viable trichomes were quantitatively detected. However, depending on the experimental conditions, it also generated artifactual viability signals. The results should help clarify the scope and limits of the MTT assay in viability analysis. |
| Published | 2020-12-31 |
| DOI | 10.1093/bbb/zbaa050 |
| PMID | 33624771 |
| MeSH | Biological Assay Colorimetry / methods* Spirulina / chemistry* Spirulina / metabolism* Tetrazolium Salts Thiazoles |
| IF | 1.516 |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-39 |