| RRC ID |
63359
|
| Author |
Khalifa HO, Majima H, Watanabe A, Kamei K.
|
| Title |
In Vitro Characterization of Twenty-One Antifungal Combinations against Echinocandin-Resistant and -Susceptible Candida glabrata.
|
| Journal |
J Fungi (Basel)
|
| Abstract |
This study was designed to analyze the interaction of 21 antifungal combinations consisting of seven major antifungal agents against 11 echinocandin- susceptible and six-resistant C. glabrata isolates. The combinations were divided into five major groups and were evaluated by checkerboard, disc diffusion, and time-killing assays. Synergy based on the fractional inhibitory concentration index of ≤0.50 was observed in 17.65-29.41% of the cases for caspofungin combinations with azoles or amphotericin B. Amphotericin B combination with azoles induced synergistic interaction in a range of 11.76-29.41%. Azole combinations and 5-flucytosine combinations with azoles or amphotericin B did not show synergistic interactions. None of the 21 combinations showed antagonistic interactions. Interestingly, 90% of the detected synergism was among the echinocandin-resistant isolates. Disk diffusion assays showed that the inhibition zones produced by antifungal combinations were equal to or greater than those produced by single drugs. The time-killing assay showed the synergistic action of caspofungin combination with fluconazole, voriconazole, and posaconazole, and the amphotericin B-5-flucytosine combination. Furthermore, for the first time, this assay confirmed the fungicidal activity of caspofungin-voriconazole and amphotericin B-5-flucytosine combinations. The combination interactions ranged from synergism to indifference and, most importantly, no antagonism was reported and most of the synergistic action was among echinocandin-resistant isolates.
|
| Volume |
7(2)
|
| Published |
2021-2-2
|
| DOI |
10.3390/jof7020108
|
| PII |
jof7020108
|
| PMID |
33540778
|
| PMC |
PMC7912999
|
| IF |
4.621
|
| Resource |
| Pathogenic eukaryotic microorganisms |
Candida glabrata IFM 60089, 61000, 61017, 61169, 61186, 61193, 61743, 61756, 62339,64652, 64679, 64684, 64686, 64689, 64903, 64905, 58273, |
