論文 - 詳細
| RRC ID | 63389 |
|---|---|
| 著者 | Goto Y, Yajima I, Kumasaka M, Ohgami N, Tanaka A, Tsuzuki T, Inoue Y, Fukushima S, Ihn H, Kyoya M, Ohashi H, Kawakami T, Bennett DC, Kato M. |
| タイトル | Transcription factor LSF (TFCP2) inhibits melanoma growth. |
| ジャーナル | Oncotarget |
| Abstract |
Late SV40 factor 3 (LSF), a transcription factor, contributes to human hepatocellular carcinoma (HCC). However, decreased expression level of LSF in skin melanoma compared to that in benign melanocytic tumors and nevi in mice and humans was found in this study. Anchorage-dependent and -independent growth of melanoma cells was suppressed by LSF overexpression through an increased percentage of G1 phase cells and an increased p21CIP1 expression level in vitro and in vivo. Anchorage-dependent growth in LSF-overexpressed melanoma cells was promoted by depletion of LSF in the LSF-overexpressed cells. Integrated results of our EMSA and chromatin immunoprecipitation assays showed binding of LSF within a 150-bp upstream region of the transcription start site of p21CIP1 in melanoma cells. Taken together, our results suggest potential roles of LSF as a growth regulator through control of the transcription of p21CIP1 in melanocytes and melanoma cells as well as a biomarker for nevus. |
| 巻・号 | 7(3) |
| ページ | 2379-90 |
| 公開日 | 2016-1-19 |
| DOI | 10.18632/oncotarget.6230 |
| PII | 6230 |
| PMID | 26506241 |
| PMC | PMC4823042 |
| MeSH | Animals Cell Line, Tumor Cell Proliferation / genetics Chromatin Immunoprecipitation Cyclin-Dependent Kinase Inhibitor p21 / genetics* DNA-Binding Proteins / biosynthesis DNA-Binding Proteins / genetics* Electrophoretic Mobility Shift Assay G1 Phase Cell Cycle Checkpoints / genetics Humans Melanocytes / metabolism Melanoma, Experimental / pathology* Mice Skin Neoplasms / pathology* Transcription Factors / biosynthesis Transcription Factors / genetics* |
| IF | 5.168 |
| リソース情報 | |
| ヒト・動物細胞 | SK-MEL-28(RCB1930) B16 melanoma(RCB1283) |