RRC ID 63393
Author Nishimura N, Ueharu H, Nishihara H, Shibuya S, Yoshida S, Higuchi M, Kanno N, Horiguchi K, Kato T, Kato Y.
Title Search for regulatory factors of the pituitary-specific transcription factor PROP1 gene.
Journal J Reprod Dev
Abstract Pituitary-specific transcription factor PROP1, a factor important for pituitary organogenesis, appears on rat embryonic day 11.5 (E11.5) in SOX2-expressing stem/progenitor cells and always coexists with SOX2 throughout life. PROP1-positive cells at one point occupy all cells in Rathke's pouch, followed by a rapid decrease in their number. Their regulatory factors, except for RBP-J, have not yet been clarified. This study aimed to use the 3 kb upstream region and 1st intron of mouse prop1 to pinpoint a group of factors selected on the basis of expression in the early pituitary gland for expression of Prop1. Reporter assays for SOX2 and RBP-J showed that the stem/progenitor marker SOX2 has cell type-dependent inhibitory and activating functions through the proximal and distal upstream regions of Prop1, respectively, while RBP-J had small regulatory activity in some cell lines. Reporter assays for another 39 factors using the 3 kb upstream regions in CHO cells ultimately revealed that 8 factors, MSX2, PAX6, PIT1, PITX1, PITX2, RPF1, SOX8 and SOX11, but not RBP-J, regulate Prop1 expression. Furthermore, a synergy effect with SOX2 was observed for an additional 10 factors, FOXJ1, HES1, HEY1, HEY2, KLF6, MSX1, RUNX1, TEAD2, YBX2 and ZFP36Ll, which did not show substantial independent action. Thus, we demonstrated 19 candidates, including SOX2, to be regulatory factors of Prop1 expression.
Volume 62(1)
Pages 93-102
Published 2016-1-1
DOI 10.1262/jrd.2015-092
PMID 26640231
PMC PMC4768783
MeSH Animals Binding Sites CHO Cells Cricetinae Cricetulus Gene Expression Regulation, Developmental* Genes, Reporter Genetic Vectors Homeodomain Proteins / metabolism* Immunohistochemistry In Situ Hybridization Introns Mice Organogenesis Pituitary Gland / metabolism Rats SOXB1 Transcription Factors / metabolism Stem Cells / cytology
IF 1.735
Human and Animal Cells CHO Tpit/F1(RCB1691)