RRC ID 63395
著者 Kida Y, Taira J, Kuwano K.
タイトル EprS, an autotransporter serine protease, plays an important role in various pathogenic phenotypes of Pseudomonas aeruginosa.
ジャーナル Microbiology (Reading)
Abstract Pseudomonas aeruginosa possesses an arsenal of both cell-associated (flagella, pili, alginate, etc.) and extracellular (exotoxin A, proteases, type III secretion effectors, etc.) virulence factors. Among them, secreted proteases that damage host tissues are considered to play an important role in the pathogenesis of P. aeruginosa infections. We previously reported that EprS, an autotransporter protease of P. aeruginosa, induces host inflammatory responses through protease-activated receptors. However, little is known about the role of EprS as a virulence factor of P. aeruginosa. In this study, to investigate whether EprS participates in the pathogenicity of P. aeruginosa, we characterized various pathogenic phenotypes of the wild-type PAO1 strain and its eprS-disrupted mutant. The growth assays demonstrated that the growth of the eprS mutant was somewhat lower than that of the wild-type strain in a minimal medium containing BSA as the sole carbon and nitrogen source. Thus, these results indicate that eprS would have a role in the growth of P. aeruginosa in the presence of limited nutrients, such as a medium containing proteinaceous materials as a sole nutrient source. Furthermore, disruption of eprS resulted in a decreased production of elastase, pigments, autoinducers and surfactants, and a reduction of swimming and swarming motilities. In addition, the eprS mutant exhibited a reduction in the ability to associate with A549 cells and an attenuation of virulence in leucopenic mice as compared with the wild-type strain. Collectively, these results suggest that EprS exerts pleiotropic effects on various pathogenic phenotypes of P. aeruginosa.
巻・号 162(2)
ページ 318-329
公開日 2016-2-1
DOI 10.1099/mic.0.000228
PMID 26678838
MeSH Animals Bacterial Adhesion / genetics Cell Line Humans Mice Pseudomonas Infections / microbiology Pseudomonas Infections / pathology* Pseudomonas aeruginosa / genetics Pseudomonas aeruginosa / growth & development Pseudomonas aeruginosa / pathogenicity* Serine Proteases / genetics* Surface-Active Agents / metabolism Virulence Factors / genetics*
IF 2.138
リソース情報
ヒト・動物細胞 A549