RRC ID 63398
Author Tachi K, Shiraishi A, Bando H, Yamashita T, Tsuboi I, Kato T, Hara H, Ohneda O.
Title FOXA1 expression affects the proliferation activity of luminal breast cancer stem cell populations.
Journal Cancer Sci
Abstract The expression of estrogen receptor is the key in most breast cancers (BC) and binding of estrogen receptor to the genome correlates to Forkhead protein (FOXA1) expression. We herein assessed the correlation between the cancer stem cell (CSC) population and FOXA1 expression in luminal BC. We established luminal BC cells derived from metastatic pleural effusion and analyzed the potency of CSC and related factors with established luminal BC cell lines. We also confirmed that mammosphere cultures have an increased aldehyde dehydrogenase-positive population, which is one of the CSC markers, compared with adherent culture cells. Using a quantitative PCR analysis, we found that mammosphere forming cells showed a higher expression of FOXA1 and stemness-related genes compared with adherent culture cells. Furthermore, the growth activity and colony-forming activity of 4-hydroxytamoxifen-treated BC cells were inhibited in a mammosphere assay. Interestingly, 4-hydroxytamoxifen-resistant cells had significantly increased FOXA1 gene expression levels. Finally, we established short hairpin RNA of FOXA1 (shFOXA1) MCF-7 cells and investigated the relationship between self-renewal potential and FOXA1 expression. As a result, we found no significant difference in the number of mammospheres but decreased colony formation in shFOXA1 MCF-7 cells compared with control. These results suggest that the expression of FOXA1 appears to be involved in the proliferation of immature BC cells rather than the induction of stemness-related genes and self-renewal potency of CSCs.
Volume 107(3)
Pages 281-9
Published 2016-3-1
DOI 10.1111/cas.12870
PMID 26708273
PMC PMC4814260
MeSH Antineoplastic Agents, Hormonal / pharmacology Breast Neoplasms Cell Proliferation* Drug Resistance, Neoplasm Female Gene Expression Hepatocyte Nuclear Factor 3-alpha / genetics Hepatocyte Nuclear Factor 3-alpha / metabolism* Humans MCF-7 Cells Neoplastic Stem Cells / physiology* Tamoxifen / analogs & derivatives Tamoxifen / pharmacology
IF 4.966
Human and Animal Cells MCF7(RCB1904)