論文 - 詳細
| RRC ID | 63401 |
|---|---|
| 著者 | Suzuki T, Kazuki Y, Oshimura M, Hara T. |
| タイトル | Highly Efficient Transfer of Chromosomes to a Broad Range of Target Cells Using Chinese Hamster Ovary Cells Expressing Murine Leukemia Virus-Derived Envelope Proteins. |
| ジャーナル | PLoS One |
| Abstract |
Microcell-mediated chromosome transfer (MMCT) is an essential step for introducing chromosomes from donor cells to recipient cells. MMCT allows not only for genetic/epigenetic analysis of specific chromosomes, but also for utilization of human and mouse artificial chromosomes (HACs/MACs) as gene delivery vectors. Although the scientific demand for genome scale analyses is increasing, the poor transfer efficiency of the current method has hampered the application of chromosome engineering technology. Here, we developed a highly efficient chromosome transfer method, called retro-MMCT, which is based on Chinese hamster ovary cells expressing envelope proteins derived from ecotropic or amphotropic murine leukemia viruses. Using this method, we transferred MACs to NIH3T3 cells with 26.5 times greater efficiency than that obtained using the conventional MMCT method. Retro-MMCT was applicable to a variety of recipient cells, including embryonic stem cells. Moreover, retro-MMCT enabled efficient transfer of MAC to recipient cells derived from humans, monkeys, mice, rats, and rabbits. These results demonstrate the utility of retro-MMCT for the efficient transfer of chromosomes to various types of target cell. |
| 巻・号 | 11(6) |
| ページ | e0157187 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0157187 |
| PII | PONE-D-16-12671 |
| PMID | 27271046 |
| PMC | PMC4896634 |
| MeSH | Animals CHO Cells Chromosomes, Artificial / genetics* Cricetinae Cricetulus Gene Products, env / genetics Gene Products, env / metabolism* Gene Transfer Techniques* Genetic Vectors / genetics Leukemia Virus, Murine / genetics Leukemia Virus, Murine / metabolism* Mice NIH 3T3 Cells Rabbits Rats |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NB2a(RCB2639) |