論文 - 詳細
| RRC ID | 63407 |
|---|---|
| 著者 | Sugasawa T, Tome Y, Takeuchi Y, Yoshida Y, Yahagi N, Sharma R, Aita Y, Ueda H, Maruyama R, Takeuchi K, Morita S, Kawamai Y, Takekoshi K. |
| タイトル | Influence of Intermittent Cold Stimulations on CREB and Its Targeting Genes in Muscle: Investigations into Molecular Mechanisms of Local Cryotherapy. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Local cryotherapy is widely used as a treatment for sports-related skeletal muscle injuries. The molecular mechanisms are unknown. To clarify these mechanisms, we applied one to three 15-min cold stimulations at 4 °C to various cell lines (in vitro), the tibialis anterior (TA) muscle (ex vivo), and mouse limbs (in vivo). In the in vitro assay, cyclic AMP (cAMP) response element binding protein 1 (CREB1) was markedly phosphorylated (p-CREB1), and the CREB-binding protein (CBP) was recruited to p-CREB-1 in response to two or three cold stimulations. In a reporter assay with the cAMP-responsive element, the signals significantly increased after two to three cold stimulations at 4 °C. In the ex vivo study, CREB-targeting genes were significantly upregulated following two or three cold stimulations. The in vivo experiment disclosed that cold stimulation of a mouse limb for 9 days significantly increased mitochondrial DNA copy number and upregulated genes involved in mitochondrial biogenesis. The results suggest that local cryotherapy increases CREB transcription and upregulates CREB-targeting genes, in a manner dependent on cold stimulation frequency and duration. This information will inform further investigations into local cryotherapy as a treatment for sports-related skeletal muscle trauma. |
| 巻・号 | 21(13) |
| 公開日 | 2020-6-28 |
| DOI | 10.3390/ijms21134588 |
| PII | ijms21134588 |
| PMID | 32605164 |
| PMC | PMC7370117 |
| MeSH | Animals Cryotherapy / methods* Cyclic AMP / metabolism* Cyclic AMP-Dependent Protein Kinases / genetics Cyclic AMP-Dependent Protein Kinases / metabolism* Male Mice Mice, Inbred CBA Muscle, Skeletal / cytology Muscle, Skeletal / metabolism Muscle, Skeletal / physiology* Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / genetics Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / metabolism* Phosphorylation Signal Transduction Transcriptional Activation |
| IF | 4.556 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |