| RRC ID |
63407
|
| 著者 |
Sugasawa T, Tome Y, Takeuchi Y, Yoshida Y, Yahagi N, Sharma R, Aita Y, Ueda H, Maruyama R, Takeuchi K, Morita S, Kawamai Y, Takekoshi K.
|
| タイトル |
Influence of Intermittent Cold Stimulations on CREB and Its Targeting Genes in Muscle: Investigations into Molecular Mechanisms of Local Cryotherapy.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Local cryotherapy is widely used as a treatment for sports-related skeletal muscle injuries. The molecular mechanisms are unknown. To clarify these mechanisms, we applied one to three 15-min cold stimulations at 4 °C to various cell lines (in vitro), the tibialis anterior (TA) muscle (ex vivo), and mouse limbs (in vivo). In the in vitro assay, cyclic AMP (cAMP) response element binding protein 1 (CREB1) was markedly phosphorylated (p-CREB1), and the CREB-binding protein (CBP) was recruited to p-CREB-1 in response to two or three cold stimulations. In a reporter assay with the cAMP-responsive element, the signals significantly increased after two to three cold stimulations at 4 °C. In the ex vivo study, CREB-targeting genes were significantly upregulated following two or three cold stimulations. The in vivo experiment disclosed that cold stimulation of a mouse limb for 9 days significantly increased mitochondrial DNA copy number and upregulated genes involved in mitochondrial biogenesis. The results suggest that local cryotherapy increases CREB transcription and upregulates CREB-targeting genes, in a manner dependent on cold stimulation frequency and duration. This information will inform further investigations into local cryotherapy as a treatment for sports-related skeletal muscle trauma.
|
| 巻・号 |
21(13)
|
| 公開日 |
2020-6-28
|
| DOI |
10.3390/ijms21134588
|
| PII |
ijms21134588
|
| PMID |
32605164
|
| PMC |
PMC7370117
|
| MeSH |
Animals
Cryotherapy / methods*
Cyclic AMP / metabolism*
Cyclic AMP-Dependent Protein Kinases / genetics
Cyclic AMP-Dependent Protein Kinases / metabolism*
Male
Mice
Mice, Inbred CBA
Muscle, Skeletal / cytology
Muscle, Skeletal / metabolism
Muscle, Skeletal / physiology*
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / genetics
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / metabolism*
Phosphorylation
Signal Transduction
Transcriptional Activation
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
