RRC ID 63419
Author Banerjee A, Basu M, Blanchard TG, Chintalacharuvu SR, Guang W, Lillehoj EP, Czinn SJ.
Title Early Molecular Events in Murine Gastric Epithelial Cells Mediated by Helicobacter pylori CagA.
Journal Helicobacter
Abstract BACKGROUND:Murine models of Helicobacter pylori infection are used to study host-pathogen interactions, but lack of severe gastritis in this model has limited its usefulness in studying pathogenesis. We compared the murine gastric epithelial cell line GSM06 to the human gastric epithelial AGS cell line to determine whether similar events occur when cultured with H. pylori.
MATERIALS AND METHODS:The lysates of cells infected with H. pylori isolates or an isogenic cagA-deficient mutant were assessed for translocation and phosphorylation of CagA and for activation of stress pathway kinases by immunoblot.
RESULTS:Phosphorylated CagA was detected in both cell lines within 60 minutes. Phospho-ERK 1/2 was present within several minutes and distinctly present in GSM06 cells at 60 minutes. Similar results were obtained for phospho-JNK, although the 54 kDa phosphoprotein signal was dominant in AGS, whereas the lower molecular weight band was dominant in GSM06 cells.
CONCLUSION:These results demonstrate that early events in H. pylori pathogenesis occur within mouse epithelial cells similar to human cells and therefore support the use of the mouse model for the study of acute CagA-associated host cell responses. These results also indicate that reduced disease in H. pylori-infected mice may be due to lack of the Cag PAI, or by differences in the mouse response downstream of the initial activation events.
Volume 21(5)
Pages 395-404
Published 2016-10-1
DOI 10.1111/hel.12300
PMID 26817586
PMC PMC4965344
MeSH Adult Animals Antigens, Bacterial / metabolism* Bacterial Proteins / metabolism* Epithelial Cells / microbiology* Epithelial Cells / physiology* Helicobacter Infections / microbiology Helicobacter Infections / pathology Helicobacter pylori / pathogenicity* Host-Pathogen Interactions* Humans Immunoblotting Mice, Inbred C57BL Models, Biological Phosphorylation Protein Kinases / metabolism Protein Processing, Post-Translational Protein Transport Signal Transduction
IF 4.0
Human and Animal Cells GSM06(RCB1779)