| RRC ID |
63422
|
| 著者 |
Yoshimura T, Hamada T, Hijioka H, Souda M, Hatanaka K, Yoshioka T, Yamada S, Tsutsui M, Umekita Y, Nakamura N, Tanimoto A.
|
| タイトル |
PCP4/PEP19 promotes migration, invasion and adhesion in human breast cancer MCF-7 and T47D cells.
|
| ジャーナル |
Oncotarget
|
| Abstract |
Purkinje cell protein (PCP) 4/peptide (PEP) 19 is expressed in Purkinje cells where it has a calmodulin-binding, anti-apoptotic function. We recently demonstrated that PCP4/PEP19 is expressed and inhibit apoptosis in human breast cancer cell lines. In the present study we investigated the role of PCP4/PEP19 in cell morphology, adhesion, migration, and invasion in MCF-7 and T47D human breast cancer cell lines. Knockdown of PCP4/PEP19 reduced the formation of filopodia-like cytoplasmic structures and vinculin expression, and enhanced E-cadherin expression. Activities of migration, invasion, and cell adhesion were also decreased after the knockdown of PCP4/PEP19 in MCF-7 and T47D cells. These results suggested that PCP4/PEP19 promotes cancer cell adhesion, migration, and invasion and that PCP4/PEP19 may be a potential target for therapeutic agents in breast cancer treatment which act by inhibiting epithelial-mesenchymal transition and enhancing apoptotic cell death.
|
| 巻・号 |
7(31)
|
| ページ |
49065-49074
|
| 公開日 |
2016-8-2
|
| DOI |
10.18632/oncotarget.7529
|
| PII |
7529
|
| PMID |
27384474
|
| PMC |
PMC5226490
|
| MeSH |
Apoptosis
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology*
Cadherins / metabolism
Cell Adhesion
Cell Count
Cell Line, Tumor
Cell Movement
Culture Media
Epithelial-Mesenchymal Transition
Female
Gene Expression Regulation, Neoplastic*
Humans
MCF-7 Cells
Neoplasm Invasiveness
Nerve Tissue Proteins / metabolism*
Polycomb Repressive Complex 1 / metabolism
Pseudopodia / metabolism
RNA, Small Interfering / metabolism
Treatment Outcome
Vinculin / metabolism
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904) |
