論文 - 詳細
| RRC ID | 63428 |
|---|---|
| 著者 | Takahashi J, Misawa M, Iwahashi H. |
| タイトル | Combined treatment with X-ray irradiation and 5-aminolevulinic acid elicits better transcriptomic response of cell cycle-related factors than X-ray irradiation alone. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:5-Aminolevulinic acid (ALA) is a precursor of the photosensitizer protoporphyrin (PpIX) used in photodynamic therapy. In our previous work, PpIX enhanced the generation of reactive oxygen species by X-ray irradiation. In this study, we evaluated the potential of ALA as an endogenous sensitizer to X-ray irradiation. METHODOLOGY:Tumor-bearing C57BL/6J mice implanted with B16-BL6 melanoma cells were subsequently treated with irradiation (3 Gy/day for 10 days; total, 30 Gy) plus local administration of 50 mg/kg ALA 24 hours prior to each irradiation (ALA-XT). Tumor-bearing mice without treatment (NT), those treated with ALA only (ALAT), and those treated with X-ray irradiation only (XT) were used as controls. RESULTS:ALA potentiated tumor suppression by X-ray irradiation. In microarray analyses using tumor tissue collected after 10 sessions of fractional irradiation, functional analysis revealed that the majority of dysregulated genes in the XT and ALA-XT groups were related to cell-cycle arrest. Finally, the XT and ALA-XT groups differed in the strength of expression, but not in the pattern of expression. CONCLUSIONS:mRNA analysis revealed that the combined use of ALA and X-ray irradiation sensitized tumors to X-ray treatment. Furthermore, the present results were consistent with ALA's tumor suppressive effects in vivo. |
| 巻・号 | 92(12) |
| ページ | 774-789 |
| 公開日 | 2016-12-1 |
| DOI | 10.1080/09553002.2016.1230240 |
| PMID | 27586078 |
| MeSH | Aminolevulinic Acid / administration & dosage* Animals Apoptosis / drug effects Apoptosis / radiation effects Cell Cycle / drug effects* Cell Cycle / radiation effects* Cell Cycle Proteins / metabolism Cell Line, Tumor Female Gene Expression Regulation, Neoplastic / drug effects Gene Expression Regulation, Neoplastic / radiation effects Mice Mice, Inbred C57BL Neoplasms, Experimental / drug therapy* Neoplasms, Experimental / metabolism* Neoplasms, Experimental / pathology Photochemotherapy / methods Radiation Tolerance / drug effects Radiation Tolerance / radiation effects Radiation-Sensitizing Agents / administration & dosage Transcriptome / radiation effects* X-Rays |
| IF | 2.368 |
| リソース情報 | |
| ヒト・動物細胞 | B16/BL6(RCB2638) |