論文 - 詳細
| RRC ID | 63438 |
|---|---|
| 著者 | Yamada D, Saiki S, Furuya N, Ishikawa K, Imamichi Y, Kambe T, Fujimura T, Ueno T, Koike M, Sumiyoshi K, Hattori N. |
| タイトル | Ethambutol neutralizes lysosomes and causes lysosomal zinc accumulation. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Ethambutol is a common medicine used for the treatment of tuberculosis, which can have serious side effects, such as retinal and liver dysfunction. Although ethambutol has been reported to impair autophagic flux in rat retinal cells, the precise molecular mechanism remains unclear. Using various mammalian cell lines, we showed that ethambutol accumulated in autophagosomes and vacuolated lysosomes, with marked Zn(2+) accumulation. The enlarged lysosomes were neutralized and were infiltrated with Zn(2+) accumulations in the lysosomes, with simultaneous loss of acidification. These results suggest that EB neutralizes lysosomes leading to insufficient autophagy, implying that some of the adverse effects associated with EB in various organs may be of this mechanism. |
| 巻・号 | 471(1) |
| ページ | 109-16 |
| 公開日 | 2016-2-26 |
| DOI | 10.1016/j.bbrc.2016.01.171 |
| PII | S0006-291X(16)30171-1 |
| PMID | 26851368 |
| MeSH | Animals Antitubercular Agents / administration & dosage* Cell Line, Tumor Dose-Response Relationship, Drug Ethambutol / administration & dosage* HeLa Cells Humans Lysosomes / drug effects Lysosomes / physiology* Lysosomes / ultrastructure Metabolic Clearance Rate / drug effects Phagosomes / drug effects Phagosomes / physiology* Phagosomes / ultrastructure Rats Zinc / pharmacokinetics* |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Huh-7 |