RRC ID 63444
Author Taura A, Taura K, Koyama Y, Yamamoto N, Nakagawa T, Ito J, Ryan AF.
Title Hair cell stereociliary bundle regeneration by espin gene transduction after aminoglycoside damage and hair cell induction by Notch inhibition.
Journal Gene Ther
Abstract Once inner ear hair cells (HCs) are damaged by drugs, noise or aging, their apical structures including the stereociliary arrays are frequently the first cellular feature to be lost. Although this can be followed by progressive loss of HC somata, a significant number of HC bodies often remain even after stereociliary loss. However, in the absence of stereocilia they are nonfunctional. HCs can sometimes be regenerated by Atoh1 transduction or Notch inhibition, but they also may lack stereociliary bundles. It is therefore important to develop methods for the regeneration of stereocilia, in order to achieve HC functional recovery. Espin is an actin-bundling protein known to participate in sterociliary elongation during development. We evaluated stereociliary array regeneration in damaged vestibular sensory epithelia in tissue culture, using viral vector transduction of two espin isoforms. Utricular HCs were damaged with aminoglycosides. The utricles were then treated with a γ-secretase inhibitor, followed by espin or control transduction and histochemistry. Although γ-secretase inhibition increased the number of HCs, few had stereociliary arrays. In contrast, 46 h after espin1 transduction, a significant increase in hair-bundle-like structures was observed. These were confirmed to be immature stereociliary arrays by scanning electron microscopy. Increased uptake of FM1-43 uptake provided evidence of stereociliary function. Espin4 transduction had no effect. The results demonstrate that espin1 gene therapy can restore stereocilia on damaged or regenerated HCs.
Volume 23(5)
Pages 415-23
Published 2016-5-1
DOI 10.1038/gt.2016.12
PII gt201612
PMID 26886463
PMC PMC4860107
MeSH Aminoglycosides / toxicity Amyloid Precursor Protein Secretases / antagonists & inhibitors Amyloid Precursor Protein Secretases / genetics Animals Cochlea / drug effects Cochlea / pathology Hair Cells, Auditory, Inner / drug effects Hair Cells, Auditory, Inner / pathology Hair Cells, Auditory, Inner / ultrastructure* Humans Mice Microfilament Proteins / genetics* Microfilament Proteins / therapeutic use Microscopy, Electron, Scanning Pyridinium Compounds / pharmacology Quaternary Ammonium Compounds / pharmacology Receptors, Notch / antagonists & inhibitors Receptors, Notch / genetics* Regeneration / genetics* Stereocilia / genetics* Stereocilia / pathology Transduction, Genetic
IF 4.128
Human and Animal Cells 293(RCB1637)