論文 - 詳細
| RRC ID | 63446 |
|---|---|
| 著者 | Hida T, Hamasaki M, Matsumoto S, Sato A, Tsujimura T, Kawahara K, Iwasaki A, Okamoto T, Oda Y, Honda H, Nabeshima K. |
| タイトル | BAP1 immunohistochemistry and p16 FISH results in combination provide higher confidence in malignant pleural mesothelioma diagnosis: ROC analysis of the two tests. |
| ジャーナル | Pathol Int |
| Abstract |
Differentiation of malignant pleural mesothelioma (MPM) from benign mesothelial proliferation remains problematic. Loss of nuclear staining of BRCA1-associated protein 1 (BAP1; detected using immunohistochemistry (IHC)) and homozygous deletion (HD) of p16 (detected using fluorescence in situ hybridization (FISH)) are useful for differentiation of MPM from reactive mesothelial hyperplasia (RMH), but the correlation between BAP1 expression loss and p16 HD has not been fully described. We performed BAP1 IHC and p16-specific FISH for 40 MPM and 20 RMH cases, and measured proportions of cells showing BAP1 expression and p16 HD for each case. The diagnostic accuracy for MPM and the cut-off values of the two methods were assessed using receiver operating characteristic (ROC) analysis. BAP1 expression loss, p16 HD and coexistence of both were present in 27 (67.5 %), 27 (67.5 %) and 17 (42.5 %) MPM cases, respectively. Three MPM cases (7.5 %) and all 20 RMH cases had neither BAP1 loss nor p16 HD. There was no correlation between the results of the two methods. Their combination showed higher sensitivity (92.5 %, 37/40) and estimated probability than BAP1 IHC and p16-specific FISH used alone. BAP1 IHC and p16-specific FISH have independent diagnostic value, and have increased reliability when used in combination, for MPM diagnosis. |
| 巻・号 | 66(10) |
| ページ | 563-570 |
| 公開日 | 2016-10-1 |
| DOI | 10.1111/pin.12453 |
| PMID | 27614970 |
| MeSH | Adult Aged Aged, 80 and over Area Under Curve Biomarkers, Tumor / analysis* Cyclin-Dependent Kinase Inhibitor p16 / analysis* Cyclin-Dependent Kinase Inhibitor p16 / biosynthesis Diagnosis, Differential Female Humans Hyperplasia / diagnosis Immunohistochemistry In Situ Hybridization, Fluorescence Lung Neoplasms / diagnosis* Male Mesothelioma / diagnosis* Mesothelioma, Malignant Middle Aged Pleural Neoplasms / diagnosis* ROC Curve Sensitivity and Specificity Tumor Suppressor Proteins / analysis* Tumor Suppressor Proteins / biosynthesis Ubiquitin Thiolesterase / analysis* Ubiquitin Thiolesterase / biosynthesis |
| IF | 2.11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Clinical guideline |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) |