| RRC ID |
63465
|
| Author |
Murakawa T, Kiger AA, Sakamaki Y, Fukuda M, Fujita N.
|
| Title |
An autophagy-dependent tubular lysosomal network synchronizes degradative activity required for muscle remodeling.
|
| Journal |
J Cell Sci
|
| Abstract |
Lysosomes are compartments for the degradation of both endocytic and autophagic cargoes. The shape of lysosomes changes with cellular degradative demands; however, there is limited knowledge about the mechanisms or significance that underlies distinct lysosomal morphologies. Here, we found an extensive tubular autolysosomal network in Drosophila abdominal muscle remodeling during metamorphosis. The tubular network transiently appeared and exhibited the capacity to degrade autophagic cargoes. The tubular autolysosomal network was uniquely marked by the autophagic SNARE protein Syntaxin17 and its formation depended on both autophagic flux and degradative function, with the exception of the Atg12 and Atg8 ubiquitin-like conjugation systems. Among ATG-deficient mutants, the efficiency of lysosomal tubulation correlated with the phenotypic severity in muscle remodeling. The lumen of the tubular network was continuous and homogeneous across a broad region of the remodeling muscle. Altogether, we revealed that the dynamic expansion of a tubular autolysosomal network synchronizes the abundant degradative activity required for developmentally regulated muscle remodeling.
|
| Volume |
133(21)
|
| Published |
2020-11-9
|
| DOI |
10.1242/jcs.248336
|
| PII |
jcs.248336
|
| PMID |
33077556
|
| PMC |
PMC7673362
|
| MeSH |
Animals
Autophagy*
Drosophila
Lysosomes*
Muscles
SNARE Proteins
|
| IF |
4.573
|
| Resource |
| Drosophila |
8428R-4
5075R-1
5373R-2
1643R-2 |
