RRC ID 63472
Author Bischoff MC, Lieb S, Renkawitz-Pohl R, Bogdan S.
Title Filopodia-based contact stimulation of cell migration drives tissue morphogenesis.
Journal Nat Commun
Abstract Cells migrate collectively to form tissues and organs during morphogenesis. Contact inhibition of locomotion (CIL) drives collective migration by inhibiting lamellipodial protrusions at cell-cell contacts and promoting polarization at the leading edge. Here, we report a CIL-related collective cell behavior of myotubes that lack lamellipodial protrusions, but instead use filopodia to move as a cohesive cluster in a formin-dependent manner. We perform genetic, pharmacological and mechanical perturbation analyses to reveal the essential roles of Rac2, Cdc42 and Rho1 in myotube migration. These factors differentially control protrusion dynamics and cell-matrix adhesion formation. We also show that active Rho1 GTPase localizes at retracting free edge filopodia and that Rok-dependent actomyosin contractility does not mediate a contraction of protrusions at cell-cell contacts, but likely plays an important role in the constriction of supracellular actin cables. Based on these findings, we propose that contact-dependent asymmetry of cell-matrix adhesion drives directional movement, whereas contractile actin cables contribute to the integrity of the migrating cell cluster.
Volume 12(1)
Pages 791
Published 2021-2-4
DOI 10.1038/s41467-020-20362-2
PII 10.1038/s41467-020-20362-2
PMID 33542237
PMC PMC7862658
MeSH Actin Cytoskeleton / metabolism Actomyosin / metabolism Animals Cadherins / metabolism Cell Movement / physiology* Drosophila Proteins / metabolism Drosophila melanogaster GTP-Binding Proteins / metabolism Intravital Microscopy Morphogenesis / physiology* Muscle Fibers, Skeletal / physiology* Pseudopodia / metabolism* rac GTP-Binding Proteins / metabolism rho GTP-Binding Proteins / metabolism
IF 12.121
Resource
Drosophila 8556R-1 8556R-3