RRC ID 63490
Author Sumii T, Nakano Y, Abe T, Nakashima K, Sera T, Kudo S.
Title The Effect of Nitric Oxide on Ammonia Decomposition in Co-cultures of Hepatocytes and Hepatic Stellate Cells.
Journal In Vitro Cell Dev Biol Anim
Abstract Hepatic functions, such as albumin secretion and ammonia metabolism, are upregulated in response to hepatocyte growth factor (HGF) produced by hepatic stellate cells (HSC), as well as nitric oxide (NO) produced by endothelial cells under shear stress. However, the simultaneous effect of HSC and NO has not been previously investigated in a tri-co-culture model containing hepatocytes with HSC and endothelial cells under shear stress. We hypothesized that NO inhibits HGF production from HSC. To test this idea, we constructed a mono-culture model of hepatocytes and a co-culture model of hepatocytes and HSC and measured ammonia decomposition and HGF production in each model under NO load. Ammonia decomposition was significantly higher in the co-culture model under 0 ppm NO load, but no significant increase was observed under NO load. In the co-culture model, HGF was produced at 1.0 ng/mL under 0 ppm NO load and 0.3 ng/mL under NO load. Ammonia decomposition was increased by 1.0 ng/mL HGF, but not by 0.3 ng/mL HGF. These results indicated that NO inhibits HGF production from HSC; consequently, the effects of NO and co-culture with HSC cannot improve hepatic function simultaneously. Instead, the simultaneous effect of 1.0 ng/mL HGF and NO may further enhance hepatic function in vitro.
Volume 52(6)
Pages 625-31
Published 2016-6-1
DOI 10.1007/s11626-016-9999-y
PII 10.1007/s11626-016-9999-y
PMID 26896067
MeSH Ammonia / metabolism* Animals Cell Line Coculture Techniques Endothelial Cells / cytology Endothelial Cells / metabolism Hepatic Stellate Cells / cytology Hepatic Stellate Cells / drug effects* Hepatic Stellate Cells / metabolism Hepatocyte Growth Factor / metabolism Hepatocytes / cytology Hepatocytes / drug effects* Hepatocytes / metabolism Nitric Oxide / pharmacology* Rats
IF 1.665
Human and Animal Cells RTH33(RCB1025)